MAP4K1 抗体 (pThr165)
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北京 101111
Quick Overview for MAP4K1 抗体 (pThr165) (ABIN358384)
抗原
See all MAP4K1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- pThr165
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特异性
- This antibody detects MAP4K1 pThr165. Predicted to cross react with Mouse (100 % Antigen Homology).
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纯化方法
- Affinity purification in a 2-step procedure with the control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
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免疫原
- This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding T165 of human MAP4K1.
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亚型
- Ig Fraction
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anti-Mitogen-Activated Protein Kinase Kinase Kinase Kinase 1 (MAP4K1) (pSer171) antibody
MAP4K1 适用: 人, 大鼠 WB, ELISA, IHC (p), FACS, IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Mitogen-Activated Protein Kinase Kinase Kinase Kinase 1 (MAP4K1) (Internal Region) antibodyMAP4K1 适用: 人, 小鼠, 大鼠 WB, ELISA, ICC, IF 宿主: 兔 Polyclonal unconjugated
anti-Mitogen-Activated Protein Kinase Kinase Kinase Kinase 1 (MAP4K1) (AA 278-377) antibodyMAP4K1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1G6 unconjugated
anti-Mitogen-Activated Protein Kinase Kinase Kinase Kinase 1 (MAP4K1) (Tyr381) antibodyMAP4K1 适用: 人 WB 宿主: 兔 Polyclonal RB58818 unconjugated
anti-Mitogen-Activated Protein Kinase Kinase Kinase Kinase 1 (MAP4K1) (N-Term) antibodyVerified MAP4K1 适用: 人 WB, ELISA, IHC 宿主: 山羊 Polyclonal unconjugated
anti-Mitogen-Activated Protein Kinase Kinase Kinase Kinase 1 (MAP4K1) (N-Term) antibodyMAP4K1 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Mitogen-Activated Protein Kinase Kinase Kinase Kinase 1 (MAP4K1) (AA 231-330) antibodyMAP4K1 适用: 人 WB, ELISA, IHC (p), FACS, IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Mitogen-Activated Protein Kinase Kinase Kinase Kinase 1 (MAP4K1) (AA 346-375) antibodyMAP4K1 适用: 人 WB 宿主: 兔 Polyclonal RB11320 unconjugated
anti-Mitogen-Activated Protein Kinase Kinase Kinase Kinase 1 (MAP4K1) (AA 278-377) antibodyMAP4K1 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-Mitogen-Activated Protein Kinase Kinase Kinase Kinase 1 (MAP4K1) (Internal Region) antibodyMAP4K1 适用: 人, 小鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated
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应用备注
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ELISA: 1/1,000. Dot Blot: 1/500.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.25 mg/mL
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缓冲液
- PBS with 0.09 % (W/V) Sodium Azide as preservative.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
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- MAP4K1 (Mitogen-Activated Protein Kinase Kinase Kinase Kinase 1 (MAP4K1))
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别名
- MAP4K1
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背景
- The c-Jun amino-terminal kinases (JNKs)/stress-activated protein kinases (SAPKs) play a crucial role in stress responses in mammalian cells. The mechanism underlying this pathway in the hematopoietic system is unclear, but it is a key in understanding the molecular basis of blood cell differentiation. We have cloned a novel protein kinase, termed hematopoietic progenitor kinase 1 (HPK1), that is expressed predominantly in hematopoietic cells, including early progenitor cells. HPK1 is related distantly to the p21(Cdc42/Rac1)-activated kinase (PAK) and yeast STE20 implicated in the mitogen-activated protein kinase (MAPK) cascade. Expression of HPK1 activates JNK1 specifically, and it elevates strongly AP-1-mediated transcriptional activity in vivo. HPK1 binds and phosphorylates MEKK1 directly, whereas JNK1 activation by HPK1 is inhibited by a dominant-negative MEKK1 or MKK4/SEK mutant. Interestingly, unlike PAK65, HPK1 does not contain the small GTPase Rac1/Cdc42-binding domain and does not bind to either Rac1 or Cdc42, suggesting that HPK1. activation is Rac1/Cdc42-independent. These results indicate that HPK1 is a novel functional activator of the JNK/SAPK signaling pathway.Synonyms: HPK1, Hematopoietic progenitor kinase, MAPK/ERK kinase kinase kinase 1, MEKKK 1, Mitogen-activated protein kinase kinase kinase kinase 1
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分子量
- 91166 Da
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基因ID
- 11184, 9606
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UniProt
- Q92918
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途径
- TCR Signaling, Signaling of Hepatocyte Growth Factor Receptor
抗原
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