Separase 抗体 (pSer1126)
Quick Overview for Separase 抗体 (pSer1126) (ABIN358223)
抗原
See all Separase (ESPL1) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- pSer1126
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特异性
- This antibody detects SEPARIN pSer1126.
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纯化方法
- Protein G Affinity Chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
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免疫原
- This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S1126 of human SEPARIN.
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亚型
- Ig Fraction
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anti-Extra Spindle Poles Like 1 (ESPL1) (AA 586-701) antibody
ESPL1 适用: 人 ELISA, WB, IHC (p) 宿主: 小鼠 Monoclonal 6H6 unconjugated
anti-Extra Spindle Poles Like 1 (ESPL1) (Tyr489) antibodyESPL1 适用: 人, 小鼠 ELISA, IHC, WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Extra Spindle Poles Like 1 (ESPL1) (pSer1126) antibodyESPL1 适用: 人 IHC (p), DB 宿主: 兔 Polyclonal RB7943 unconjugated
anti-Extra Spindle Poles Like 1 (ESPL1) (AA 767-816) antibodyESPL1 适用: 人, 小鼠 ELISA, IHC, WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Extra Spindle Poles Like 1 (ESPL1) (pSer801) antibodyESPL1 适用: 人 IHC, WB, IF, IC 宿主: 兔 Polyclonal unconjugated
anti-Extra Spindle Poles Like 1 (ESPL1) (pSer801) antibodyESPL1 适用: 人 ELISA, IHC, WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Extra Spindle Poles Like 1 (ESPL1) (AA 1292-1495) antibodyESPL1 适用: 人 ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Extra Spindle Poles Like 1 (ESPL1) antibodyESPL1 适用: 小鼠 WB 宿主: 山羊 Polyclonal unconjugated
anti-Extra Spindle Poles Like 1 (ESPL1) (pSer1126) antibodyESPL1 适用: 人 IF 宿主: 兔 Polyclonal unconjugated
anti-Extra Spindle Poles Like 1 (ESPL1) (pSer801) antibodyESPL1 适用: 人, 小鼠 ELISA, IHC, WB, IF 宿主: 兔 Polyclonal unconjugated
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应用备注
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ELISA: 1/1,000. Dot Blot: 1/500. Immunohistochemistry: 1/50approx. 1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.25 mg/mL
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缓冲液
- PBS with 0.09 % (W/V) Sodium Azide as preservative.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
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- Separase (ESPL1) (Extra Spindle Poles Like 1 (ESPL1))
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别名
- Separin / ESPL1
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背景
- The metaphase-to-anaphase transition is the final discrete event in duplication and separation of the genetic material of the cell. Its timing is regulated by the activation of the anaphase-promoting complex (APC). In both budding and fission yeast, the degradation of the Pds1 or Cut2 protein, respectively, is required for the onset of sister chromatid separation. Both proteins are APC substrates. Pds1 and Cut2 proteins associate with the yeast separin proteins Esp1 and Cut1, respectively, and prevent the separins from promoting chromatid separation. Pds1 and Cut2 are also called anaphase inhibitors or securinsSynonyms: Caspase-like protein ESPL1, ESP1, Extra spindle poles-like 1 protein, KIAA0165, Separase
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分子量
- 233113 Da
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基因ID
- 9700, 9606
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UniProt
- Q14674
抗原
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