CDC6 抗体 (pSer54)
Quick Overview for CDC6 抗体 (pSer54) (ABIN358103)
抗原
See all CDC6 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- pSer54
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特异性
- This antibody detects CDC6 pSer54.
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纯化方法
- Protein G Affinity Chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
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免疫原
- This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S54 of human Cdc6.
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亚型
- Ig Fraction
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anti-Cell Division Cycle 6 Homolog (S. Cerevisiae) (CDC6) (AA 1-560) antibody
CDC6 适用: 人 WB, IF, PLA 宿主: 兔 Polyclonal unconjugated
anti-Cell Division Cycle 6 Homolog (S. Cerevisiae) (CDC6) (AA 1-326) antibodyCDC6 适用: 人, 小鼠 WB, IF, IP 宿主: 兔 Polyclonal unconjugated
anti-Cell Division Cycle 6 Homolog (S. Cerevisiae) (CDC6) (AA 1-141) antibodyCDC6 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Cell Division Cycle 6 Homolog (S. Cerevisiae) (CDC6) antibodyCDC6 适用: 人, 小鼠, 大鼠 WB, IHC (p), IF (p) 宿主: 兔 Polyclonal unconjugated
anti-Cell Division Cycle 6 Homolog (S. Cerevisiae) (CDC6) (AA 147-549) antibodyCDC6 适用: 人, 大鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Cell Division Cycle 6 Homolog (S. Cerevisiae) (CDC6) (AA 434-533) antibodyCDC6 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-Cell Division Cycle 6 Homolog (S. Cerevisiae) (CDC6) (Internal Region) antibodyVerified CDC6 适用: 人 WB, ELISA 宿主: 山羊 Polyclonal unconjugated
anti-Cell Division Cycle 6 Homolog (S. Cerevisiae) (CDC6) (pSer54) antibodyCDC6 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal RB7524 unconjugated
anti-Cell Division Cycle 6 Homolog (S. Cerevisiae) (CDC6) (N-Term), (pSer54) antibodyCDC6 适用: 人, 小鼠, 大鼠, 猴 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Cell Division Cycle 6 Homolog (S. Cerevisiae) (CDC6) (AA 20-69), (pSer54) antibodyCDC6 适用: 人, 小鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
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应用备注
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ELISA: 1/1,000. Western Blot: 1/100-1/500. Immunohistochemistry: 1/50-1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.25 mg/mL
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缓冲液
- PBS with 0.09 % (W/V) Sodium Azide as preservative.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
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- CDC6 (Cell Division Cycle 6 Homolog (S. Cerevisiae) (CDC6))
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别名
- CDC6
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背景
- The protein encoded by this gene is highly similar to Saccharomyces cerevisiae Cdc6, a protein essential for the initiation of DNA replication. This protein functions as a regulator at the early steps of DNA replication. It localizes in cell nucleus during cell cyle G1, but translocates to the cytoplasm at the start of S phase. The subcellular translocation of this protein during cell cyle is regulated through its phosphorylation by Cdks. Transcription of this protein was reported to be regulated in response to mitogenic signals through transcriptional control mechanism involving E2F proteins.Synonyms: CDC18L, CDC6-related protein, Cdc18-related protein, Cell division control protein 6 homolog, HsCDC6, HsCdc18, p62(cdc6)
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分子量
- 62720 Da
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基因ID
- 990, 9606
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UniProt
- Q99741
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途径
- DNA Replication, Synthesis of DNA
抗原
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