Caspase 6 抗体 (pSer257)
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Quick Overview for Caspase 6 抗体 (pSer257) (ABIN358091)
抗原
See all Caspase 6 (CASP6) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- pSer257
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特异性
- This antibody detects Caspase-6 pSer257. Predicted to cross react with Mouse (100 % Antigen Homology).
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纯化方法
- Protein G Affinity Chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
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免疫原
- This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S257 of human caspase 6.
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亚型
- Ig Fraction
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anti-Caspase 6, Apoptosis-Related Cysteine Peptidase (CASP6) (AA 194-293) antibody
CASP6 适用: Cow WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Caspase 6, Apoptosis-Related Cysteine Peptidase (CASP6) (AA 81-179) antibodyCASP6 适用: Cow WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Caspase 6, Apoptosis-Related Cysteine Peptidase (CASP6) (AA 194-293) antibodyCASP6 适用: 人 ELISA, ICC, FACS 宿主: 小鼠 Monoclonal 3B7E10 unconjugated
anti-Caspase 6, Apoptosis-Related Cysteine Peptidase (CASP6) (AA 24-44), (N-Term) antibodyCASP6 适用: 人 WB, IHC, FACS 宿主: 兔 Polyclonal unconjugated
anti-Caspase 6, Apoptosis-Related Cysteine Peptidase (CASP6) (AA 7-27) antibodyCASP6 适用: 小鼠, 大鼠 WB, IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Caspase 6, Apoptosis-Related Cysteine Peptidase (CASP6) (AA 81-179) antibodyCASP6 适用: Cow WB, IHC 宿主: 小鼠 Monoclonal C1 unconjugated
anti-Caspase 6, Apoptosis-Related Cysteine Peptidase (CASP6) (AA 7-27), (N-Term) antibodyCASP6 适用: 小鼠, 大鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Caspase 6, Apoptosis-Related Cysteine Peptidase (CASP6) (AA 151-250) antibodyCASP6 适用: 人 ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Caspase 6, Apoptosis-Related Cysteine Peptidase (CASP6) (AA 24-293) antibodyCASP6 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Caspase 6, Apoptosis-Related Cysteine Peptidase (CASP6) (Cleaved-Asp179) antibodyCASP6 适用: 人, 大鼠 WB, IHC, ELISA, ICC, IF 宿主: 兔 Polyclonal unconjugated
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应用备注
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ELISA: 1/1,000. Western Blot: 1/100-1/500. Immunohistochemistry: 1/50-1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.25 mg/mL
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缓冲液
- PBS with 0.09 % (W/V) Sodium Azide as preservative.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
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- Caspase 6 (CASP6) (Caspase 6, Apoptosis-Related Cysteine Peptidase (CASP6))
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别名
- Caspase-6
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背景
- Caspase 6 is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme. This protein could be processed by caspases 7, 8 and 10, and is thought to function as a downstream enzyme in the caspase activation cascade.Synonyms: Apoptotic protease Mch-2, CASP-6, CASP6, MCH-2, MCH2
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分子量
- 33310 Da
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基因ID
- 839, 9606
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UniProt
- P55212
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途径
- Apoptosis, Caspase Cascade in Apoptosis
抗原
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