Fibrillarin 抗体 (Middle Region)
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Quick Overview for Fibrillarin 抗体 (Middle Region) (ABIN357938)
抗原
See all Fibrillarin (FBL) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- Middle Region
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特异性
- This antibody detects FBL (Center).
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纯化方法
- Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
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免疫原
- This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the center region of human FBL.
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亚型
- Ig Fraction
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应用备注
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ELISA: 1/1,000. Western Blot: 1/50-1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.25 mg/mL
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缓冲液
- PBS with 0.09 % (W/V) Sodium Azide as preservative.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
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- Fibrillarin (FBL)
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别名
- FBL
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背景
- FBL is a component of a nucleolar small nuclear ribonucleoprotein (snRNP) particle thought to participate in the first step in processing preribosomal RNA. It is associated with the U3, U8, and U13 small nuclear RNAs and is located in the dense fibrillar component (DFC) of the nucleolus. This protein contains an N-terminal repetitive domain that is rich in glycine and arginine residues, like fibrillarins in other species. Its central region resembles an RNA-binding domain and contains an RNP consensus sequence. Antisera from approximately 8 % of humans with the autoimmune disease scleroderma recognize fibrillarin.Synonyms: FIB1, FLRN, Fibrillarin, LOT3, NOP1, Nucleolar Marker, Nucleolar protein 1, RNU3IP1, U3 small nucleolar RNA-associated protein NOP1, rRNA 2'-O-methyltransferase fibrillarin
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分子量
- 33784 Da predicted from amino acid sequence. Theoretical pI: 10.18
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基因ID
- 2091, 9606
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UniProt
- P22087
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途径
- Ribonucleoside Biosynthetic Process
抗原
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