OCT4 抗体 (N-Term)
Quick Overview for OCT4 抗体 (N-Term) (ABIN357427)
抗原
See all OCT4 (POU5F1) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- N-Term
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特异性
- This antibody detects OCT4 (OCT3 at N-term.
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交叉反应 （详细）
- Species reactivity (tested):Human
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纯化方法
- Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS
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免疫原
- This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human 39724.
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亚型
- Ig Fraction
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anti-POU Class 5 Homeobox 1 (POU5F1) (AA 81-164) antibody
POU5F1 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 1B11 unconjugated
anti-POU Class 5 Homeobox 1 (POU5F1) antibodyKO Validated POU5F1 适用: 人 WB, IF, ICC, IP, FACS, IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-POU Class 5 Homeobox 1 (POU5F1) (AA 201-300) antibodyPOU5F1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-POU Class 5 Homeobox 1 (POU5F1) (AA 1-360) antibodyPOU5F1 适用: 人 WB, ELISA, IHC, IF, FACS 宿主: 小鼠 Monoclonal 2H41D8 unconjugated
anti-POU Class 5 Homeobox 1 (POU5F1) antibodyPOU5F1 适用: 人 WB, ELISA, ICC, FACS 宿主: 小鼠 Monoclonal 7E7 unconjugated
anti-POU Class 5 Homeobox 1 (POU5F1) (AA 136-360) antibodyPOU5F1 适用: 人 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-POU Class 5 Homeobox 1 (POU5F1) (AA 1-360) antibodyPOU5F1 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-POU Class 5 Homeobox 1 (POU5F1) (AA 81-164) antibodyPOU5F1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1D2 unconjugated
anti-POU Class 5 Homeobox 1 (POU5F1) (Internal Region) antibodyPOU5F1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-POU Class 5 Homeobox 1 (POU5F1) antibodyPOU5F1 适用: 人, 猴 WB, IF, IHC (p) 宿主: 兔 Polyclonal unconjugated
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应用备注
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ELISA 1: 1,000. Western blot 1: 100 - 1: 500. Immunofluorescence 1: 50 - 1: 100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.25 mg/mL
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缓冲液
- PBS with 0.09 % (W/V) sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer.
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- OCT4 (POU5F1) (POU Class 5 Homeobox 1 (POU5F1))
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别名
- OCT3/4
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背景
- Transcription factors containing the POU homeodomain have been shown to be important regulators of tissue-specific gene expression in lymphoid and pituitary differentiation and in early mammalian development. Two forms of OCT3 mRNA are expressed in adult tissues as a result of alternative splicing--OCT3A and OCT3B. Reverse transcriptase PCR showed low level of expression in both OCT3A and OCT3B mRNA in all adult human tissues examined. Oct3 is present in mouse oocytes before and after fertilization. When fertilized oocytes were injected with antisense Oct3 oligonucleotides or double-stranded DNA containing the octamer motif, embryonic DNA synthesis was inhibited and the embryos were arrested at the one-cell stage.Synonyms: OCT3, OCT4, OTF3, Oct-3, Oct-4, Octamer-binding transcription factor 3, POU domain, POU5F1, class 5, transcription factor 1
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分子量
- 38562 Da
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基因ID
- 5460, 9606
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UniProt
- Q01860
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途径
- Stem Cell Maintenance
抗原
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