CD33 抗体 (N-Term)
Quick Overview for CD33 抗体 (N-Term) (ABIN357141)
抗原
See all CD33 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 18-48, N-Term
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特异性
- This antibody detects CD33 (SIGLEC3) at N-term.
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交叉反应 （详细）
- Species reactivity (tested):Human.
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纯化方法
- Protein G Chromatography, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
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免疫原
- This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 18~48 amino acids from the N-terminal region of Human CD33/SIGLEC3.
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亚型
- Ig Fraction
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anti-CD33 (CD33) (AA 101-200) antibody
CD33 适用: 人, 小鼠 FACS, WB, ELISA, IHC (fro), ICC, IF (p), IF (cc), IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-CD33 (CD33) (AA 15-237) antibodyCD33 适用: 人 FACS, WB, ELISA, IHC 宿主: 小鼠 Monoclonal 2C6B7 unconjugated
anti-CD33 (CD33) (AA 15-237) antibodyCD33 适用: 人 FACS, ELISA, IHC 宿主: 小鼠 Monoclonal 2C6B7 unconjugated
anti-CD33 (CD33) (AA 18-240) antibodyCD33 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-CD33 (CD33) (AA 49-259) antibodyCD33 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-CD33 (CD33) (AA 261-364) antibodyCD33 适用: 人 FACS, WB, ELISA, IHC (fro), IF (p), IF (cc), IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-CD33 (CD33) (AA 1-364) antibodyCD33 适用: 人 WB, ELISA, RNAi 宿主: 小鼠 Monoclonal 2D12-G4 unconjugated
anti-CD33 (CD33) antibodyCD33 适用: 人, 小鼠, 大鼠 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-CD33 (CD33) antibodyCD33 适用: 人, Non-Human Primate FACS, WB, IP, IHC (fro), ICC, Func, MS 宿主: 小鼠 Monoclonal WM53 unconjugated
anti-CD33 (CD33) antibodyCD33 适用: 人, Non-Human Primate FACS, WB, IP, IHC (fro), ICC, MS 宿主: 小鼠 Monoclonal WM53 unconjugated
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应用备注
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ELISA: 1/1,000. Western blot: 1/100-1/500. Immunohistochemistry: 1/50-1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.25 mg/mL
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缓冲液
- PBS with 0.09 % (W/V) Sodium Azide as preservative.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
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- CD33
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别名
- CD33 / SIGLEC3
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背景
- CD33/SIGLEC3 is a putative adhesion molecule of myelomonocytic-derived cells that mediates sialic-acid dependent binding to cells. It preferentially binds to alpha2,6-linked sialic acid, the sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. In the immune response, SIGLEC3 may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules. This protein Induces apoptosis in acute myeloid leukemia (in vitro). It has been shown to interact with PTPN6/SHP-1 and PTPN11/SHP-2 upon phosphorylation. SIGLEC3 expresses in monocytic/myeloid lineage cells, and contains 2 copies of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in downmodulation of cellular responses. The phosphorylated ITIM motif binds to the SH2 domain of PTPN6/SHP-1. Phosphorylation of Tyr-340 is involved in binding to PTPN6 and PTPN11. Phosphorylation of Tyr-358 is involved in binding to PTPN6. The gene for SIGLEC3 belongs to the immunoglobulin superfamily.Synonyms: Myeloid cell surface antigen CD33, Sialic acid-binding Ig-like lectin 3, Siglec-3, gp67
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基因ID
- 945, 9606
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UniProt
- P20138
抗原
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