Nanog 抗体 (Middle Region)
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北京 101111
Quick Overview for Nanog 抗体 (Middle Region) (ABIN357015)
抗原
See all Nanog (NANOG) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 101-131, Middle Region
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特异性
- This antibody detects NANOG at Center.
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纯化方法
- Protein A Chromatography eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
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免疫原
- This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the center region (aa101-131) of human NANOG.
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亚型
- Ig Fraction
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anti-Nanog Homeobox (NANOG) antibody
NANOG 适用: 人 WB, ELISA, IF, IHC, ICC, IHC (p), IP, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Nanog Homeobox (NANOG) (AA 101-200) antibodyNANOG 适用: 人, 小鼠, 大鼠 WB, ELISA, FACS, ICC, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Nanog Homeobox (NANOG) (AA 1-305) antibodyNANOG 适用: 人 WB, ELISA, IF, FACS, ICC 宿主: 小鼠 Monoclonal 8A1D11 unconjugated
anti-Nanog Homeobox (NANOG) (AA 71-170) antibodyNANOG 适用: 人, 小鼠 WB, ELISA, FACS, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Nanog Homeobox (NANOG) (AA 15-49) antibodyNANOG 适用: 人 WB, ELISA, IF, IHC, FACS 宿主: 兔 Polyclonal unconjugated
anti-Nanog Homeobox (NANOG) (Internal Region) antibodyVerified NANOG 适用: 人 WB, ELISA, IF, IHC 宿主: 山羊 Polyclonal unconjugated
anti-Nanog Homeobox (NANOG) (AA 98-195) antibodyNANOG 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 2C11 unconjugated
anti-Nanog Homeobox (NANOG) (AA 98-195) antibodyNANOG 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 3A12 unconjugated
anti-Nanog Homeobox (NANOG) (AA 20-166) antibodyNANOG 适用: 人 WB, ELISA, ICC 宿主: 小鼠 Monoclonal 1E6C4 unconjugated
anti-Nanog Homeobox (NANOG) (AA 267-292) antibodyNANOG 适用: 人 WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated
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应用备注
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ELISA: 1/1,000. Western blot: 1/50-1/100. Immunohistochemistry on paraffin sections: 1/10-1/50. Immunofluorescence: 1/10 -1/50.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.25 mg/mL
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缓冲液
- PBS with 0.09 % (W/V) Sodium Azide as preservative.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
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- Nanog (NANOG) (Nanog Homeobox (NANOG))
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别名
- NANOG
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背景
- NANOG is a Ttranscription regulator involved in inner cell mass and embryonic stem (ES) cels proliferation and self-renewal. It imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. This protein blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes. NANOG acts as a transcriptional activator or repressor. It binds optimally to the DNA consensus sequence 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3'. When overexpressed, this protein promotes cells to enter into S phase and proliferation.Synonyms: Homeobox protein NANOG
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分子量
- 34489 Da
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基因ID
- 79923, 9606
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UniProt
- Q9H9S0
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途径
- Stem Cell Maintenance
抗原
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