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Nanog 抗体 (N-Term)

This 兔 多克隆 anti-Nanog antibody specifically detects Nanog in WB, IF, FACS, IHC (p) 和 EIA. The antibody is reactive with 人 samples.
产品编号 ABIN357014
发货至: 中国
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Quick Overview for Nanog 抗体 (N-Term) (ABIN357014)

抗原

See all Nanog (NANOG) 抗体
Nanog (NANOG) (Nanog Homeobox (NANOG))

适用

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宿主

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克隆类型

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多克隆

标记

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This Nanog antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)
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    AA 25-54, N-Term

    特异性

    This antibody detects NANOG at N-term.

    纯化方法

    Prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS

    免疫原

    KLH conjugated synthetic peptide between 25~54 amino acids from the N-terminal region of Human NANOG.

    亚型

    Ig Fraction
  • 应用备注

    ELISA 1: 1,000. Western blot 1: 50 - 1: 100.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    0.25 mg/mL

    缓冲液

    PBS, 0.09 % (W/V) Sodium Azide

    储存液

    Sodium azide

    注意事项

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Avoid repeated freezing and thawing.

    储存条件

    4 °C/-20 °C

    储存方法

    Store undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
  • 抗原

    Nanog (NANOG) (Nanog Homeobox (NANOG))

    别名

    NANOG

    背景

    NANOG is a transcription regulator involved in inner cell mass and embryonic stem (ES) cell proliferation and self-renewal. It imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. This protein blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes. NANOG acts as a transcriptional activator or repressor. It binds optimally to the DNA consensus sequence 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3'. When overexpressed, this protein promotes cells to enter into S phase and proliferation.Synonyms: Homeobox protein NANOG

    分子量

    34489 Da

    基因ID

    79923, 9606

    UniProt

    Q9H9S0

    途径

    Stem Cell Maintenance
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