Nanog 抗体 (N-Term)
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北京 101111
Quick Overview for Nanog 抗体 (N-Term) (ABIN357014)
抗原
See all Nanog (NANOG) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 25-54, N-Term
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特异性
- This antibody detects NANOG at N-term.
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纯化方法
- Prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS
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免疫原
- KLH conjugated synthetic peptide between 25~54 amino acids from the N-terminal region of Human NANOG.
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亚型
- Ig Fraction
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应用备注
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ELISA 1: 1,000. Western blot 1: 50 - 1: 100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.25 mg/mL
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缓冲液
- PBS, 0.09 % (W/V) Sodium Azide
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
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- Nanog (NANOG) (Nanog Homeobox (NANOG))
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别名
- NANOG
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背景
- NANOG is a transcription regulator involved in inner cell mass and embryonic stem (ES) cell proliferation and self-renewal. It imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. This protein blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes. NANOG acts as a transcriptional activator or repressor. It binds optimally to the DNA consensus sequence 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3'. When overexpressed, this protein promotes cells to enter into S phase and proliferation.Synonyms: Homeobox protein NANOG
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分子量
- 34489 Da
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基因ID
- 79923, 9606
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UniProt
- Q9H9S0
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途径
- Stem Cell Maintenance
抗原
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