UBA2 抗体 (C-Term)
Quick Overview for UBA2 抗体 (C-Term) (ABIN356597)
抗原
See all UBA2 抗体适用
宿主
克隆类型
标记
应用范围
-
-
抗原表位
- C-Term
-
特异性
- This antibody is specific to UBA2 (C-term).
-
纯化方法
- Protein G Chromatography, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
-
免疫原
- This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human UBA2.
-
亚型
- Ig Fraction
-
-
anti-SUMO1 Activating Enzyme Subunit 2 (UBA2) (AA 449-564) antibody
UBA2 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC, FACS, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-SUMO1 Activating Enzyme Subunit 2 (UBA2) antibodyUBA2 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-SUMO1 Activating Enzyme Subunit 2 (UBA2) (N-Term) antibodyVerified UBA2 适用: 人, 小鼠 WB, ELISA, IF 宿主: 山羊 Polyclonal unconjugated
anti-SUMO1 Activating Enzyme Subunit 2 (UBA2) (C-Term) antibodyUBA2 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-SUMO1 Activating Enzyme Subunit 2 (UBA2) (AA 1-640) antibodyUBA2 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated
anti-SUMO1 Activating Enzyme Subunit 2 (UBA2) (N-Term) antibodyUBA2 适用: 人 WB, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-SUMO1 Activating Enzyme Subunit 2 (UBA2) antibodyUBA2 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-SUMO1 Activating Enzyme Subunit 2 (UBA2) (C-Term) antibodyUBA2 适用: 人 WB, IHC, IF, IC 宿主: 兔 Polyclonal unconjugated
anti-SUMO1 Activating Enzyme Subunit 2 (UBA2) antibodyUBA2 适用: 人, 大鼠, 犬, 猴 WB, IHC, IF 宿主: 小鼠 Monoclonal 4A3 unconjugated
anti-SUMO1 Activating Enzyme Subunit 2 (UBA2) antibodyUBA2 适用: 大鼠 WB 宿主: 兔 Polyclonal unconjugated
-
-
应用备注
-
ELISA: 1/1,000. Western blot: 1/100approx. 1/500. Immunohistochemistry: 1/50approx. 1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
-
-
-
状态
- Liquid
-
浓度
- 0.25 mg/mL
-
缓冲液
- PBS containing 0.09 % (W/V) Sodium Azide as preservative.
-
储存液
- Sodium azide
-
注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
注意事项
- Avoid repeated freezing and thawing.
-
储存条件
- 4 °C/-20 °C
-
储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
-
-
- UBA2 (SUMO1 Activating Enzyme Subunit 2 (UBA2))
-
别名
- SAE2 / UBA2
-
背景
- Ubiquitin is covalently attached to target proteins by a multienzymatic system consisting of E1 (ubiquitin-activating), E2 (ubiquitin-conjugating), and E3 (ubiquitin-ligating) enzymes. NEDD8, a ubiquitin-like protein, is conjugated to proteins in a manner analogous to ubiquitinylation. beta-amyloid precursor protein-binding protein-1 (APPBP1) can bind to NEDD8 in rabbit reticulocyte lysates. However, since APPBP1 shows similarity to only the N-terminal domain of an E1 enzyme, it must interact with a protein showing similarity to the C-terminal region of E1s. By searching sequence databases, a cDNAs encoding UBA3 was identified as the human homolog of yeast Uba3. The predicted 442-amino acid UBA3 protein shares 43 % sequence identity with yeast Uba3. In vitro, UBA3 formed a complex with APPBP1 and a thioester linkage with NEDD8. APPBP1/UBA3 complex may function as an E1-like enzyme for the activation of NEDD8.Synonyms: ARX, Anthracycline-associated resistance ARX, HRIHFB2115, SUMO-activating enzyme subunit 2, UBLE1B, Ubiquitin-like 1-activating enzyme E1B
-
基因ID
- 10054, 9606
-
UniProt
- Q9UBT2
抗原
-
