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Nanog 抗体

This anti-Nanog antibody is a 小鼠 单克隆 antibody detecting Nanog in WB, IHC (p) 和 EIA. Suitable for 人.
产品编号 ABIN356386
发货至: 中国
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中国
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
4A Biotech Co.,Ltd.
Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for Nanog 抗体 (ABIN356386)

抗原

See all Nanog (NANOG) 抗体
Nanog (NANOG) (Nanog Homeobox (NANOG))

适用

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宿主

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小鼠

克隆类型

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单克隆

标记

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This Nanog antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

克隆位点

60CT70-5-12
  • 特异性

    This antibody is specific to Human NANOG.

    交叉反应 (详细)

    Species reactivity (tested):Human.

    产品特性

    Synonyms: Homeobox protein NANOG

    纯化方法

    Protein G Chromatography, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS

    免疫原

    This antibody is generated from mouse immunized with human recombinant NANOGprotein.

    亚型

    IgG1
  • 应用备注

    ELISA: 1/1,000. Western blot: 1/100-1/500.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    0.25 mg/mL

    缓冲液

    PBS, 0.09 % (W/V) Sodium Azide

    储存液

    Sodium azide

    注意事项

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C/-20 °C

    储存方法

    Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
    Shelf life: one year from despatch.

    有效期

    12 months
  • 抗原

    Nanog (NANOG) (Nanog Homeobox (NANOG))

    别名

    NANOG

    背景

    NANOG is a transcription regulator involved in inner cell mass and embryonic stem (ES) cell proliferation and self-renewal. It imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. This protein blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes. NANOG acts as a transcriptional activator or repressor. It binds optimally to the DNA consensus sequence 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3'. When overexpressed, this protein promotes cells to enter into S phase and proliferation.Synonyms: Homeobox protein NANOG

    分子量

    34620 Da

    基因ID

    79923

    UniProt

    Q9H9S0

    途径

    Stem Cell Maintenance
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