Keratin 10 抗体
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北京 101111
Quick Overview for Keratin 10 抗体 (ABIN356290)
抗原
See all Keratin 10 (KRT10) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
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This antibody recognises Cytokeratin 10+13 (CK10+13). It reacts with 53 kDa and 56.6 kDa cytokeratin (CK) proteins by Western blot. With frozen sections, this antibody serves as a differentiation related marker for all stratified epithelia and stains all suprabasal cell in both cornifying and non-cornifying stratified epithelia and more differentiated cells of squamous carcinomas.
Cellular Localization: Cytoplasmic. -
交叉反应 （详细）
- Also reacts to: KRT13
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产品特性
- Synonyms: Cytokeratin-10, CK10, CK-10, Keratin-10, KRT10, KPP, K10, Keratin, Type I Cytoskeletal 10
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纯化方法
- Purified
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免疫原
- Cytokeratin extraction from human ectocervical epithelium. Remarks: Molecular weight of antigen: CK10 is 56. 9 kDa and CK13 is 53 kDa.
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亚型
- IgG2a
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anti-Keratin 10 (KRT10) (AA 153-456) antibody
KRT10 适用: 人 IHC, WB, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Keratin 10 (KRT10) antibodyKRT10 适用: 人, 小鼠, 大鼠, 犬, 斑马鱼 IHC, WB, FACS, ICC, IHC (fro) 宿主: 小鼠 Monoclonal RKSE60 unconjugated
anti-Keratin 10 (KRT10) (AA 345-454) antibodyKRT10 适用: 人, 小鼠, 大鼠, 猴 IHC, WB, FACS, ELISA 宿主: 小鼠 Monoclonal 3C2F5 unconjugated
anti-Keratin 10 (KRT10) (AA 1-584) antibodyKRT10 适用: 人 IHC, WB, IF, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Keratin 10 (KRT10) antibodyKRT10 适用: 人, 小鼠, 大鼠, 犬, Pig IHC, WB, FACS, ICC, IHC (fro) 宿主: 小鼠 Monoclonal RKSE60 unconjugated
anti-Keratin 10 (KRT10) antibodyKRT10 适用: 人, 小鼠, 大鼠 WB, IHC (p), IF (cc) 宿主: 兔 Monoclonal 1D8 unconjugated
anti-Keratin 10 (KRT10) antibodyKRT10 适用: 人 IHC 宿主: 小鼠 Monoclonal 610M unconjugated Recombinant Antibody
anti-Keratin 10 (KRT10) (Internal Region) antibodyKRT10 适用: 人, 小鼠, 大鼠 IHC, WB, IF, ELISA, ICC 宿主: 兔 Polyclonal unconjugated
anti-Keratin 10 (KRT10) antibodyKRT10 适用: 人, 小鼠 IHC, WB, StM 宿主: 小鼠 Monoclonal LH2 unconjugated
anti-Keratin 10 (KRT10) antibodyKRT10 适用: 人 IHC (p), ICC, IP 宿主: 小鼠 Monoclonal VIK-10 unconjugated
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应用备注
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Suitable for Immunohistochemistry and Immunocytochemistry (Frozen tissue sections onlyor cell smears). For IHC dilute concentared antibody at 1/50-1/100, use streptavidinapprox. biotin system orpolymer system, incubate 30 minutes at room temperature.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 0.2 mg/mL
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缓冲液
- PBS, pH 7.4 containing 1 % BSA as stabilizer and 0.05 % Sodium Azide as preservative.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C/-20 °C
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储存方法
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Storethe antibody undiluted at 2-8 °Cfor one month or (in aliquots) at -28 °Cfor longer. Avoid repeated freezing and thawing.
Shelf life: One year from despatch. -
有效期
- 12 months
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- Keratin 10 (KRT10)
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别名
- Cytokeratin 10
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背景
- Cytokeratins are intermediate filament keratins found in the intracytoplasmic cytoskeleton of epithelial tissue There are two types of Cytokeratins: the low weight, acidic type I cytokeratins and the high weight, basic or neutral type II. Cytokeratins are usually found in pairs comprising a type I Cytokeratin and a type II cytokeratin. The high molecular weight cytokeratins, which are the basic or neutral cytokeratins, comprise subtypes CK1, CK2, CK3, CK4, CK5, CK6, CK7, CK8 and CK9. The low molecular weight cytokeratins, which are the acidic cytokeratins, comprise subtypes CK10, CK12, CK 13, CK14, CK16, CK17, CK18, CK19 and CK20. Recently Silveira et. al. (3) have reported that in tongue squamous cell carcinoma the expression of CK 10 was significantly correlated with the presence of metasis.Synonyms: CK-10, CK10, Cytokeratin-10, K10, KPP, KRT10, Keratin, Keratin-10, Type I Cytoskeletal 10
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基因ID
- 3858
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UniProt
- P13645
抗原
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