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HMGB1 抗体 (N-Term)

The 小鼠 单克隆 anti-HMGB1 antibody (Clone J2E1) (ABIN356226) specifically detects HMGB1 in WB, IHC (p) 和 EIA. The antibody is reactive with 人 samples.
产品编号 ABIN356226
发货至: 中国
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北京 101111
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Room 801-803
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Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for HMGB1 抗体 (N-Term) (ABIN356226)

抗原

See all HMGB1 抗体
HMGB1 (High Mobility Group Box 1 (HMGB1))

适用

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宿主

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小鼠

克隆类型

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单克隆

标记

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This HMGB1 antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

克隆位点

J2E1
  • 抗原表位

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    AA 1-215, N-Term

    特异性

    The antibody recognizes human HMGB1. Other species not tested.

    产品特性

    Synonyms: High mobility group protein 1, HMG1, HMG-1, High mobility group protein B1, HMGB-1,Amphoterin

    纯化方法

    Protein-G affinity chromatography

    免疫原

    Recombinant human HMGB1 (1-215aa) purified from High Five (Trichoplusia ni) insect cells.

    亚型

    IgG2b
  • 应用备注

    ELISA. Western blot (1: 500 - 1: 2,000), recommended starting dilution is 1: 1,000. Immunohistochemistry (1: 100 - 1: 300), recommended starting dilution is 1: 200.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    1.0 mg/mL

    缓冲液

    PBS, pH 7.4, containing 0.09 % sodium azide

    储存液

    Sodium azide

    注意事项

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C/-20 °C

    储存方法

    Store the antibody undiluted at 2-8 °C for up to two weeks or (in aliquots) at -20 °C or -70 °Cfor longer. Avoid repeated freezing and thawing.
    Shelf life: one year from despatch.

    有效期

    12 months
  • 抗原

    HMGB1 (High Mobility Group Box 1 (HMGB1))

    别名

    HMGB1

    背景

    High mobility group box1 protein (HMGB1) is a very abundant chromatin-binding protein residing in the eukaryotic cell nucleus and acting in the assembly of nucleoprotein complexes. Inside the cell, HMGB1 binds to DNA and has a role in transcriptional regulation. Outside the cell, HMGB1 acts as a cytokine and has activities that resemble those of tumor necrosis factor.Synonyms: Amphoterin, HMG-1, HMG1, HMGB-1, High mobility group protein 1, High mobility group protein B1

    基因ID

    3146

    UniProt

    P09429

    途径

    p53 Pathway, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Toll-Like Receptors Cascades, Smooth Muscle Cell Migration, Inflammasome
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