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IL7R 抗体 (pTyr449)

IL7R 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
产品编号 ABIN351553
发货至: 中国
  • 抗原 See all IL7R 抗体
    IL7R (Interleukin 7 Receptor (IL7R))
    抗原表位
    • 24
    • 16
    • 9
    • 7
    • 6
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    pTyr449
    适用
    • 110
    • 50
    • 26
    • 7
    • 4
    • 2
    • 1
    人, 小鼠, 大鼠
    宿主
    • 81
    • 36
    • 28
    • 1
    • 1
    克隆类型
    • 81
    • 64
    • 2
    多克隆
    标记
    • 63
    • 11
    • 10
    • 8
    • 4
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This IL7R antibody is un-conjugated
    应用范围
    • 85
    • 77
    • 45
    • 26
    • 26
    • 13
    • 13
    • 9
    • 8
    • 8
    • 5
    • 3
    • 2
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    品牌
    IHC-plus™
    特异性
    A BLAST analysis was used to suggest cross-reactivity with IL-7R from human, mouse and rat sources based on 100 % homology with the immunizing sequence. Cross-reactivity with IL-7R from other sources has not been determined.
    纯化方法
    Immunoaffinity purified
    免疫原
    Synthetic peptide corresponding to residues near Y449 of mouse IL-7 protein.

    Type of Immunogen: Synthetic peptide
    Top Product
    Discover our top product IL7R Primary Antibody
  • 应用备注
    Approved: ELISA (1:100000), IHC, IHC-P (5 μg/mL), WB (1:1000 - 1:10000)

    Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Expect a band approximately 55 kD in size corresponding to phosphorylated IL-7 in the appropriate cell lysate or extract. This phospho-specific polyclonal antibody reacts with mouse IL-7 pY449 and shows minimal reactivity by ELISA against the non-phosphorylated form of the immunizing peptide.
    说明

    Target Species of Antibody: Mouse

    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    Lot specific
    缓冲液
    0.02 M potassium phosphate, 0.15 M sodium chloride,  pH 7.2, 0.01 % sodium azide.
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    注意事项
    Avoid repeated freezing and thawing.
    储存条件
    4 °C,-20 °C
    储存方法
    4°C or -20°C, Avoid freeze-thaw cycles.
  • 抗原
    IL7R (Interleukin 7 Receptor (IL7R))
    别名
    IL7R / CD127 (IL7R 产品)
    别名
    IL7R antibody, CD127 antibody, IL-7Ralpha antibody, CDW127 antibody, IL-7R-alpha antibody, IL7RA antibody, ILRA antibody, IL-7RA antibody, interleukin 7 receptor antibody, IL7R antibody, Il7r antibody
    背景
    Name/Gene ID: IL7R
    Family: Interleukin

    Synonyms: IL7R, CD127, CD127 antigen, IL-7RA, IL-7 receptor subunit alpha, IL7RA, ILRA, Interleukin 7 receptor, CDW127, IL-7R subunit alpha, IL-7R-alpha
    基因ID
    3575
    UniProt
    P16871
    途径
    JAK/STAT Signaling, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Regulation of Cell Size
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