IL7R 抗体 (C-Term)
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Quick Overview for IL7R 抗体 (C-Term) (ABIN351552)
抗原
See all IL7R 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- C-Term
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特异性
- A BLAST analysis was used to suggest cross-reactivity with IL-7 from human, mouse and rat sources based on 100 % homology with the immunizing sequence. Cross-reactivity with IL-7 from other sources has not been determined
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纯化方法
- Immunoaffinity purified
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免疫原
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Synthetic peptide corresponding to a region near the carboxy terminus of mouse IL-7 protein.
Type of Immunogen: Synthetic peptide
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应用备注
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Approved: ELISA (1:500 - 1:50000), IHC, IHC-P (5 μg/mL), WB (1:1000 - 1:10000)
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Expect a band approximately 55 kD in size corresponding to IL-7 in the appropriate cell lysate or extract. -
说明
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Target Species of Antibody: Mouse
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- Lot specific
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缓冲液
- 0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- avoid freeze thaw cycles. Store undiluted.
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储存条件
- 4 °C,-20 °C
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储存方法
- Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Store undiluted.
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- IL7R (Interleukin 7 Receptor (IL7R))
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别名
- IL7R / CD127
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背景
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Name/Gene ID: IL7R
Family: Interleukin
Synonyms: IL7R, CD127, CD127 antigen, IL-7RA, IL-7 receptor subunit alpha, IL7RA, ILRA, Interleukin 7 receptor, CDW127, IL-7R subunit alpha, IL-7R-alpha -
基因ID
- 3575
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UniProt
- P16871
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途径
- JAK/STAT Signaling, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Regulation of Cell Size
抗原
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