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IL7R 抗体 (C-Term)

The 兔 多克隆 anti-IL7R antibody (ABIN351552) specifically detects IL7R in WB, ELISA, IHC 和 IHC (p). The antibody is reactive with 人, 小鼠 和 大鼠 samples.
产品编号 ABIN351552
发货至: 中国
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Quick Overview for IL7R 抗体 (C-Term) (ABIN351552)

抗原

See all IL7R 抗体
IL7R (Interleukin 7 Receptor (IL7R))

适用

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人, 小鼠, 大鼠

宿主

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克隆类型

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多克隆

标记

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This IL7R antibody is un-conjugated

应用范围

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Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
  • 抗原表位

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    C-Term

    特异性

    A BLAST analysis was used to suggest cross-reactivity with IL-7 from human, mouse and rat sources based on 100 % homology with the immunizing sequence. Cross-reactivity with IL-7 from other sources has not been determined

    纯化方法

    Immunoaffinity purified

    免疫原

    Synthetic peptide corresponding to a region near the carboxy terminus of mouse IL-7 protein.

    Type of Immunogen: Synthetic peptide
  • 应用备注

    Approved: ELISA (1:500 - 1:50000), IHC, IHC-P (5 μg/mL), WB (1:1000 - 1:10000)

    Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Expect a band approximately 55 kD in size corresponding to IL-7 in the appropriate cell lysate or extract.

    说明

    Target Species of Antibody: Mouse

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    Lot specific

    缓冲液

    0.02 M potassium phosphate, 0.15 M sodium chloride,  pH 7.2, 0.01 % sodium azide.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    avoid freeze thaw cycles. Store undiluted.

    储存条件

    4 °C,-20 °C

    储存方法

    Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Store undiluted.
  • 抗原

    IL7R (Interleukin 7 Receptor (IL7R))

    别名

    IL7R / CD127

    背景

    Name/Gene ID: IL7R
    Family: Interleukin

    Synonyms: IL7R, CD127, CD127 antigen, IL-7RA, IL-7 receptor subunit alpha, IL7RA, ILRA, Interleukin 7 receptor, CDW127, IL-7R subunit alpha, IL-7R-alpha

    基因ID

    3575

    UniProt

    P16871

    途径

    JAK/STAT Signaling, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Regulation of Cell Size
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