NEDD8 抗体 (N-Term)
Quick Overview for NEDD8 抗体 (N-Term) (ABIN7698970)
抗原
See all NEDD8 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- N-Term
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原理
- Sheep antibody to NEDD8
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特异性
- Specific for NEDD8.
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交叉反应
- 人, 小鼠, 大鼠
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交叉反应 （详细）
- Other species not yet tested.
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纯化方法
- IgG
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免疫原
- A synthetic peptide from the n-terminal of mouse NEDD8 (Ubiquitin-like protein Nedd8 Neddylin) conjugated to immunogenic carrier protein has been used as the immunogen. The antigen is homologous in human rat zebrafish drosophila and xenopus.
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亚型
- IgG
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anti-Neural Precursor Cell Expressed, Developmentally Down-Regulated 8 (NEDD8) antibody
NEDD8 适用: 人 WB, IHC, ELISA, ICC, FACS 宿主: 小鼠 Monoclonal 5B8 unconjugated
anti-Neural Precursor Cell Expressed, Developmentally Down-Regulated 8 (NEDD8) antibodyNEDD8 适用: 人 IHC, ELISA, ICC, FACS 宿主: 小鼠 Monoclonal 1A7 unconjugated
anti-Neural Precursor Cell Expressed, Developmentally Down-Regulated 8 (NEDD8) antibodyNEDD8 适用: 人, 小鼠, 大鼠, 猴 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Neural Precursor Cell Expressed, Developmentally Down-Regulated 8 (NEDD8) (N-Term) antibodyNEDD8 适用: 人, 小鼠, 大鼠, 猴, Cow, 非洲爪蟾 WB, IHC, IF, IHC (p), IP, FACS 宿主: 兔 Polyclonal unconjugated
anti-Neural Precursor Cell Expressed, Developmentally Down-Regulated 8 (NEDD8) antibodyNEDD8 适用: 人 WB, IF, IHC (p), FACS, EIA 宿主: 小鼠 Monoclonal 1A7 unconjugated
anti-Neural Precursor Cell Expressed, Developmentally Down-Regulated 8 (NEDD8) (AA 1-81) antibodyNEDD8 适用: 人 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Neural Precursor Cell Expressed, Developmentally Down-Regulated 8 (NEDD8) (N-Term) antibodyNEDD8 适用: 人, 小鼠, 大鼠, 猴 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Neural Precursor Cell Expressed, Developmentally Down-Regulated 8 (NEDD8) (AA 1-32) antibodyNEDD8 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Neural Precursor Cell Expressed, Developmentally Down-Regulated 8 (NEDD8) (N-Term) antibodyNEDD8 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Neural Precursor Cell Expressed, Developmentally Down-Regulated 8 (NEDD8) antibodyNEDD8 适用: 人 WB, IHC, ELISA, IF, IHC (p), FACS 宿主: 小鼠 Monoclonal 5B8 unconjugated
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应用备注
- IHC (both on frozen,paraffin embedded tissues) WB. Use at a concentration of 10-50,micro,g,ml. The optimal dilution should be determined by the end user. Not yet tested in other applications.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Reconstitute in 500 µl of sterile water. Centrifuge to remove any insoluble material.
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注意事项
- Avoid freeze and thaw cycles.
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储存条件
- 4 °C,-20 °C
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储存方法
- Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
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有效期
- 12 months
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- NEDD8 (Neural Precursor Cell Expressed, Developmentally Down-Regulated 8 (NEDD8))
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别名
- NEDD8
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背景
- Function: Ubiquitin-like protein which plays an important role in cell cycle control and embryogenesis. Covalent attachment to its substrates requires prior activation by the E1 complex UBE1C-APPBP1 and linkage to the E2 enzyme UBE2M. Attachment of NEDD8 to cullins activates their associated E3 ubiquitin ligase activity and thus promotes polyubiquitination and proteasomal degradation of cyclins and other regulatory protein. Subcellular location: Mainly nuclear Tissue specificity: Ubiquitously expressed (at protein level). Post-translational modification: Cleavage of precursor form by UCHL3 or SENP8 is necessary for function
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UniProt
- P29595
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途径
- Ubiquitin Proteasome Pathway
抗原
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