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GAPDH 抗体 (C-Term)

Name: GAPDH 抗体 (C-Term)
Brand: antibodies-online
SKU: ABIN350330
Availability: OutOfStock

GAPDH 适用: 大鼠 WB, IHC 宿主: 绵羊 Polyclonal unconjugated

WB on human neutrophil lysate using Sheep antibody to c-terminal of GAPDH (Glyceraldehyde 3 phosphate dehydrogenase): IgG (ABIN350330) at a concentration of 20 µg/ml. (GAPDH 抗体 (C-Term))

1 image

产品编号 ABIN350330

发货至: 中国

Datasheet as PDF

抗原 See all GAPDH 抗体

GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))
抗原表位
33

22

13

13

7

6

6

5

4

4

3

3

3

3

3

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
C-Term
适用
213

146

128

64

46

39

25

25

23

19

19

17

15

15

14

13

10

9

8

6

4

4

4

4

4

4

4

3

3

2

2

2

2

1

1

1

1

1

1
大鼠
宿主
160

88

16

8

4
绵羊
克隆类型
180

94
多克隆
标记
157

29

18

16

3

3

3

3

3

3

3

2

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1
This GAPDH antibody is un-conjugated
应用范围
221

103

72

64

38

33

30

27

16

15

15

15

7

6

5

4

3

3

1

1

1

1

1

1
Western Blotting (WB), Immunohistochemistry (IHC)

原理

Sheep antibody to GAPDH

特异性

Specific for GAPDH.

交叉反应

人, 小鼠, 大鼠

交叉反应（详细）

Other species not yet tested.

纯化方法

IgG

免疫原

A synthetic peptide from c-terminal of rat GAPDH conjugated to blue carrier protein was used as the antigen. The antigen is homologous in mouse and shares 90% identity with human sequence.

亚型

IgG

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anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (AA 62-91), (N-Term) antibody
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anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (AA 154-324) antibody
GAPDH 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (AA 2-148) antibody
GAPDH 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (AA 3-335) antibody
GAPDH 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated

应用备注

IHC WB. A concentration of 10-50 μg,ml is recommended. The optimal concentration should be determined by the end user. Not yet tested in other applications.

限制

仅限研究用
状态

Lyophilized

溶解方式

Reconstitute in 500 µL of sterile water. Centrifuge to remove any insoluble material.

注意事项

Avoid freeze and thaw cycles.

储存条件

4 °C,-20 °C

储存方法

Maintain the lyophilised/reconstituted antibodies frozen at -20C for long term storage and refrigerated at 2-8C for a shorter term. When reconstituting glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

有效期

12 months
抗原

GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

别名

GAPDH (GAPDH 产品)

背景

FUNCTION: May bind tRNA. Has uracil-DNA-glycosylase activity. May activate transcription in neurons. Functions as a killing protein in apoptosis when over-expressed. CATALYTIC ACTIVITY: D-glyceraldehyde 3-phosphate + phosphate + NAD+ = 3-phospho-D-glyceroyl phosphate + NADH. PATHWAY: Carbohydrate degradation, glycolysis, pyruvate from D-glyceraldehyde 3-phosphate: step 1/5. SUBUNIT: Homotetramer. SUBCELLULAR LOCATION: Cytoplasm. TISSUE SPECIFICITY: High levels in skeletal muscle and heart low levels in liver brain and kidney.

UniProt

P04797