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GAPDH 抗体

Cited in 1 publication. This anti-GAPDH antibody is a 兔 多克隆 antibody detecting GAPDH in WB 和 IHC. Suitable for 大鼠.
产品编号 ABIN350328
发货至: 中国
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中国
北京 101111
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Room 801-803
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Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for GAPDH 抗体 (ABIN350328)

抗原

See all GAPDH 抗体
GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

适用

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大鼠

宿主

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克隆类型

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多克隆

标记

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This GAPDH antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (IHC)
  • 原理

    Rabbit antibody to GAPDH

    特异性

    Specific for GAPDH.

    交叉反应

    人, Marmoset, 小鼠, 大鼠

    交叉反应 (详细)

    Other species not yet tested.

    纯化方法

    IgG

    免疫原

    A synthetic peptide from rat GAPDH conjugated to blue carrier protein was used as the antigen. The antigen is homologous in mouse and shares 90% identity with human sequence.

    亚型

    IgG
  • 应用备注

    IHC WB. A concentration of 1-5,micro,g,ml is recommended. The optimal concentration should be determined by the end user. Not yet tested in other applications.

    限制

    仅限研究用
  • 状态

    Lyophilized

    溶解方式

    Reconstitute in 500 µl of sterile water. Centrifuge to remove any insoluble material.

    注意事项

    Avoid freeze and thaw cycles.

    储存条件

    4 °C,-20 °C

    储存方法

    Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

    有效期

    12 months
  • Gengenbacher, Müller-Rudorf, Poggio, Gräßel, Dumit, Kreutmair, Lippert, Duyster, Illert: "Proteomic Phosphosite Analysis Identified Crucial NPM-ALK-Mediated NIPA Serine and Threonine Residues." in: International journal of molecular sciences, Vol. 20, Issue 16, (2020) (PubMed).

  • 抗原

    GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

    别名

    GAPDH

    背景

    FUNCTION: May bind tRNA. Has uracil-DNA-glycosylase activity. May activate transcription in neurons. Functions as a killing protein in apoptosis when over-expressed. CATALYTIC ACTIVITY: D-glyceraldehyde 3-phosphate + phosphate + NAD+ = 3-phospho-D-glyceroyl phosphate + NADH. PATHWAY: Carbohydrate degradation, glycolysis, pyruvate from D-glyceraldehyde 3-phosphate: step 1/5. SUBUNIT: Homotetramer. SUBCELLULAR LOCATION: Cytoplasm. TISSUE SPECIFICITY: High levels in skeletal muscle and heart low levels in liver brain and kidney.

    UniProt

    P04797
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