GAPDH 抗体
Quick Overview for GAPDH 抗体 (ABIN350328)
抗原
See all GAPDH 抗体适用
宿主
克隆类型
标记
应用范围
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原理
- Rabbit antibody to GAPDH
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特异性
- Specific for GAPDH.
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交叉反应
- 人, Marmoset, 小鼠, 大鼠
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交叉反应 (详细)
- Other species not yet tested.
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纯化方法
- IgG
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免疫原
- A synthetic peptide from rat GAPDH conjugated to blue carrier protein was used as the antigen. The antigen is homologous in mouse and shares 90% identity with human sequence.
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亚型
- IgG
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应用备注
- IHC WB. A concentration of 1-5,micro,g,ml is recommended. The optimal concentration should be determined by the end user. Not yet tested in other applications.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Reconstitute in 500 µl of sterile water. Centrifuge to remove any insoluble material.
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注意事项
- Avoid freeze and thaw cycles.
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储存条件
- 4 °C,-20 °C
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储存方法
- Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
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有效期
- 12 months
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: "Proteomic Phosphosite Analysis Identified Crucial NPM-ALK-Mediated NIPA Serine and Threonine Residues." in: International journal of molecular sciences, Vol. 20, Issue 16, (2020) (PubMed).
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: "Proteomic Phosphosite Analysis Identified Crucial NPM-ALK-Mediated NIPA Serine and Threonine Residues." in: International journal of molecular sciences, Vol. 20, Issue 16, (2020) (PubMed).
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- GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))
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别名
- GAPDH
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背景
- FUNCTION: May bind tRNA. Has uracil-DNA-glycosylase activity. May activate transcription in neurons. Functions as a killing protein in apoptosis when over-expressed. CATALYTIC ACTIVITY: D-glyceraldehyde 3-phosphate + phosphate + NAD+ = 3-phospho-D-glyceroyl phosphate + NADH. PATHWAY: Carbohydrate degradation, glycolysis, pyruvate from D-glyceraldehyde 3-phosphate: step 1/5. SUBUNIT: Homotetramer. SUBCELLULAR LOCATION: Cytoplasm. TISSUE SPECIFICITY: High levels in skeletal muscle and heart low levels in liver brain and kidney.
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UniProt
- P04797
抗原
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