GRP78 抗体
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北京 101111
Quick Overview for GRP78 抗体 (ABIN349665)
抗原
See all GRP78 (HSPA5) 抗体适用
宿主
克隆类型
标记
应用范围
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原理
- Anti-BiP | Lumenal-binding protein (rabbit antibody)
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交叉反应 (详细)
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Anacardium occidentale, Arabidopsis thaliana, Brassica napus,Chara australis R.Br, Chlamydomonas reinhardtii, Cucumis sativus, Mangifera indica, Moniliophthora perniciosa, Nicotiana benthamiana, Nicotiana tabacum, Raphanus sativa L. Tokinashi-daikon, Olea europaea, Oryza sativa, Picea abies, Pistachio sp., Physcomitrium patens, Schinus molle, Spinacia oleracea, Solanum lycopersicum, Solanum tuberosum, Triticum aestivum, Zea mays
Not reactive in: Ostreococcus tauri, Schizosaccharomyces pombe -
预测反应
- Arabis alpina,Capsella rubella,Capsicum annuum,Citrus clementina,Citrus sinsensis,Eucalyptus grandis,Glycine max,Hordeum vulgare,Isatis tincorina,Prunus persica,Triticum aestivium,Petunia hybrida,Picea sitcHensis,Populus trichocarpa,Ricinus comminus,Vitis vinifera
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纯化方法
- Immunogen affinity purified serum
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免疫原
- KLH-conjugated synthetic peptide derived from Arabidopsis thaliana BiP proteins: BiP1 At5g28540 Q9LKR3
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亚型
- IgG
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应用备注
- 1 : 8000 (ELISA), 1 : 600 (IF), 1 : 2000 (WB)
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说明
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Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- For reconstitution add 50 μL of sterile water
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缓冲液
- Immunogen affinity purified serum in PBS pH 7.4.
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注意事项
- Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube
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储存条件
- -20 °C
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储存方法
- Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
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: "Identification of glycosyltransferases involved in cell wall synthesis of wheat endosperm." in: Journal of proteomics, Vol. 78, pp. 508-21, (2013) (PubMed).
: "Protocol: Optimised methodology for isolation of nuclei from leaves of species in the Solanaceae and Rosaceae families." in: Plant methods, Vol. 9, Issue 1, pp. 31, (2013) (PubMed).
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: "Identification of glycosyltransferases involved in cell wall synthesis of wheat endosperm." in: Journal of proteomics, Vol. 78, pp. 508-21, (2013) (PubMed).
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- GRP78 (HSPA5) (Heat Shock 70kDa Protein 5 (Glucose-Regulated Protein, 78kDa) (HSPA5))
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别名
- BiP
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背景
- BiP2 (Binding immunoglobulin protein) is localized in endoplasmic reticulum lumen (ER) and plays a role in protein assembly inside ER. BiP protein is abundant under all growth conditions but its synthesis can increase under conditions that lead to the accumulation of unfolded polypeptides in endoplasmic reticulum (ER).
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分子量
- 73.5 | 80 kDa
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基因ID
- 834207
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UniProt
- Q39043
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途径
- Thyroid Hormone Synthesis, ER-Nucleus Signaling
抗原
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