GRP78 抗体
Quick Overview for GRP78 抗体 (ABIN349665)
抗原
See all GRP78 (HSPA5) 抗体适用
宿主
克隆类型
标记
应用范围
-
-
产品特性
- Expected / apparent Molecular Weight of the Antigene: 73.5 / 80 kDa
-
纯化方法
- affinity purified
-
免疫原
- KLH-conjugated synthetic peptide derived from Arabidopsis thaliana BiP proteins: BiP1 At5g28540 Q9LKR3, BiP2 At5g42020 F4K007 , BiP3 At5g09080 Q8H1B3
-
亚型
- IgG
-
-
-
-
应用备注
- Recommended Dilution: 1 : 8000 (ELISA), 1 : 2000 with standard ECL (WB), 1 : 600 ( IF).
-
说明
-
Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel.
-
限制
- 仅限研究用
-
-
-
状态
- Lyophilized
-
溶解方式
- For reconstitution add 200 µL of sterile water
-
缓冲液
- PBS pH 7.4
-
注意事项
- Make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
-
储存条件
- -20 °C
-
-
-
: "Identification of glycosyltransferases involved in cell wall synthesis of wheat endosperm." in: Journal of proteomics, Vol. 78, pp. 508-21, (2013) (PubMed).
: "Protocol: Optimised methodology for isolation of nuclei from leaves of species in the Solanaceae and Rosaceae families." in: Plant methods, Vol. 9, Issue 1, pp. 31, (2013) (PubMed).
-
: "Identification of glycosyltransferases involved in cell wall synthesis of wheat endosperm." in: Journal of proteomics, Vol. 78, pp. 508-21, (2013) (PubMed).
-
- GRP78 (HSPA5) (Heat Shock 70kDa Protein 5 (Glucose-Regulated Protein, 78kDa) (HSPA5))
-
别名
- BiP
-
背景
-
AGI Code: At5g42020, At5g42020
BiP2 (Binding immunoglobulin protein) is localized in endoplasmic reticulum lumen (ER) and plays a role in protein assembly inside ER. BiP protein is abundant under all growth conditions but its synthesis can increase under conditions that lead to the accumulation of unfolded polypeptides in endoplasmic reticulum (ER). -
分子量
- expected: 73.5 kDa, apparent: 80 kDa
-
UniProt
- Q39043
-
途径
- Thyroid Hormone Synthesis, ER-Nucleus Signaling
抗原
-