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GRP78 抗体

Cited in 2+ publications. The 兔 多克隆 anti-GRP78 antibody (ABIN349665) specifically detects GRP78 in WB, ELISA, IF 和 EM. The antibody is reactive with Spinach, Arabidopsis thaliana, Zea mays, Physcomytrium Patens, Cashew nut, Tomato, Triticum aestivum, Olea europaea, Chlamydomonas reinhardtii, Cucumis sativus, Mangifera indica, Moniliophthora perniciosa, Nicotiana benthamiana, Nicotiana tabacum, Chara australis, Oryza sativa, Picea abies, Pistachio, Potato, Radish, Rape 和 Schinus molle samples.
产品编号 ABIN349665
发货至: 中国
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中国
北京 101111
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Beijing Economic Technological Development Area
Room 801-803
4A Biotech Co.,Ltd.
Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for GRP78 抗体 (ABIN349665)

抗原

See all GRP78 (HSPA5) 抗体
GRP78 (HSPA5) (Heat Shock 70kDa Protein 5 (Glucose-Regulated Protein, 78kDa) (HSPA5))

适用

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Spinach, Arabidopsis thaliana, Zea mays, Physcomytrium Patens, Cashew nut, Tomato, Triticum aestivum, Olea europaea, Chlamydomonas reinhardtii, Cucumis sativus, Mangifera indica, Moniliophthora perniciosa, Nicotiana benthamiana, Nicotiana tabacum, Chara australis, Oryza sativa, Picea abies, Pistachio, Potato, Radish, Rape, Schinus molle

宿主

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克隆类型

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多克隆

标记

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This GRP78 antibody is un-conjugated

应用范围

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Western Blotting (WB), ELISA, Immunofluorescence (IF), Electron Microscopy (EM)
  • 原理

    Anti-BiP | Lumenal-binding protein (rabbit antibody)

    交叉反应 (详细)

    Anacardium occidentale, Arabidopsis thaliana, Brassica napus,Chara australis R.Br, Chlamydomonas reinhardtii, Cucumis sativus, Mangifera indica, Moniliophthora perniciosa, Nicotiana benthamiana, Nicotiana tabacum, Raphanus sativa L. Tokinashi-daikon, Olea europaea, Oryza sativa, Picea abies, Pistachio sp., Physcomitrium patens, Schinus molle, Spinacia oleracea, Solanum lycopersicum, Solanum tuberosum, Triticum aestivum, Zea mays
    Not reactive in: Ostreococcus tauri, Schizosaccharomyces pombe

    预测反应

    Arabis alpina,Capsella rubella,Capsicum annuum,Citrus clementina,Citrus sinsensis,Eucalyptus grandis,Glycine max,Hordeum vulgare,Isatis tincorina,Prunus persica,Triticum aestivium,Petunia hybrida,Picea sitcHensis,Populus trichocarpa,Ricinus comminus,Vitis vinifera

    纯化方法

    Immunogen affinity purified serum

    免疫原

    KLH-conjugated synthetic peptide derived from Arabidopsis thaliana BiP proteins: BiP1 At5g28540 Q9LKR3

    亚型

    IgG
  • 应用备注

    1 : 8000 (ELISA), 1 : 600 (IF), 1 : 2000 (WB)

    说明

    Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel.

    限制

    仅限研究用
  • 状态

    Lyophilized

    溶解方式

    For reconstitution add 50 μL of sterile water

    缓冲液

    Immunogen affinity purified serum in PBS pH 7.4.

    注意事项

    Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

    储存条件

    -20 °C

    储存方法

    Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
  • Suliman, Chateigner-Boutin, Francin-Allami, Partier, Bouchet, Salse, Pont, Marion, Rogniaux, Tessier, Guillon, Larré: "Identification of glycosyltransferases involved in cell wall synthesis of wheat endosperm." in: Journal of proteomics, Vol. 78, pp. 508-21, (2013) (PubMed).

    Sikorskaite, Rajamäki, Baniulis, Stanys, Valkonen: "Protocol: Optimised methodology for isolation of nuclei from leaves of species in the Solanaceae and Rosaceae families." in: Plant methods, Vol. 9, Issue 1, pp. 31, (2013) (PubMed).

  • 抗原

    GRP78 (HSPA5) (Heat Shock 70kDa Protein 5 (Glucose-Regulated Protein, 78kDa) (HSPA5))

    别名

    BiP

    背景

    BiP2 (Binding immunoglobulin protein) is localized in endoplasmic reticulum lumen (ER) and plays a role in protein assembly inside ER. BiP protein is abundant under all growth conditions but its synthesis can increase under conditions that lead to the accumulation of unfolded polypeptides in endoplasmic reticulum (ER).

    分子量

    73.5 | 80 kDa

    基因ID

    834207

    UniProt

    Q39043

    途径

    Thyroid Hormone Synthesis, ER-Nucleus Signaling
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