PCK1 抗体
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北京 101111
Quick Overview for PCK1 抗体 (ABIN349649)
抗原
See all PCK1 抗体适用
宿主
克隆类型
标记
应用范围
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原理
- Anti-PEPC | Phosphoenolpyruvate carboxylase
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交叉反应 （详细）
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Ananas comosus, Arabidopsis thaliana, Cenchrus ciliaris, Chenopodium quinoa, Chloris gayana, Chromera velia, Cyanthobasis fruticulosa, Glycine max, Hordeum vulgare, Jatropha curcas, Kochia prostrata, Leptochloa fusca, Lupinus sp. , Megathyrsus maximus, Mesembryanthemum crystallinum, Nicotiana tabacum, Oryza sativa, Panicum antidotale, Panicum coloratum, Petrosimonia nigdeensis, Pinus strobus, Saccharum spp. hybrid clone C91-301, Salsola lanata, Salsola laricifolia,Salsola grandis, Salsola tragus Sorghum bicolor, Synechocystis PCC 6803, Phaeodactylum tricornutum (strain CCAP 1055/1), Pinus strobus, Thalassiosira weissfloggi, Zea mays, Zostera muelleri
Not reactive in: Methanothermobacter thermautotrophicus -
预测反应
- Amaranthus sp.,Brassica napus,Cucumis sativus (PEPC1,PEPC2,PEPC3),Flaveria bidentis,Flaveria trinervia,Lupinus albus,Mammillaria thornberi,Manihot esculenta,Manihot obovata,Medicago sativa,Morinda citrifolia,Nannochloropsis gaditana CCMP526,Nopalea gaumeri,Opuntia macbridei,Pachycereus pringlei,Pachycereus hollianus,Pisum sativa,Phaseolus vulgaris,Phragmites australis,Populus sp.,Saccharum spp,Solanum tuberosum,Spinacia oleracea,Streptanthus tortuosus,Triticum aestivum,algae,diatoms: Thalassiosira pseudonana,other species: Salmonella sp.,Schiedea hookeri,Shigella sp. Schiedea sarmentosa,Solanum lycopersicum,Streptanthus farnsworthianus,Tacinga saxatilis,Yersinia sp. Vibrio sp.,Quercus sp.
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纯化方法
- Immunogen affinity purified serum
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免疫原
- KLH-conjugated synthetic peptide well conserved PEPC1 and sequences from different plant species including Arabidopsis thaliana Q9MAH0
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anti-phosphoenolpyruvate Carboxykinase 1 (Soluble) (PCK1) (C-Term) antibody
PCK1 适用: 人 WB, IP, IF 宿主: 兔 Polyclonal unconjugated
anti-phosphoenolpyruvate Carboxykinase 1 (Soluble) (PCK1) (AA 592-622), (C-Term) antibodyPCK1 适用: 人, 小鼠, 大鼠 WB, IHC (p), IF 宿主: 兔 Polyclonal RB03646 unconjugated
anti-phosphoenolpyruvate Carboxykinase 1 (Soluble) (PCK1) (AA 1-622) antibodyPCK1 适用: 人 WB, ELISA, IP, IF 宿主: 小鼠 Monoclonal 3E4 unconjugated
anti-phosphoenolpyruvate Carboxykinase 1 (Soluble) (PCK1) (Middle Region) antibodyPCK1 适用: 人, 小鼠, 大鼠, 犬, Cow, 兔, 马, Pig, 豚鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-phosphoenolpyruvate Carboxykinase 1 (Soluble) (PCK1) (AA 312-563) antibodyPCK1 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-phosphoenolpyruvate Carboxykinase 1 (Soluble) (PCK1) (Middle Region) antibodyPCK1 适用: 人, 小鼠, 大鼠, 犬, Cow, 兔, 马, Pig, 豚鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-phosphoenolpyruvate Carboxykinase 1 (Soluble) (PCK1) (C-Term) antibodyPCK1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-phosphoenolpyruvate Carboxykinase 1 (Soluble) (PCK1) (AA 330-379) antibodyPCK1 适用: 人, 小鼠, 大鼠, Cow, 兔, 马, 猴, 小鸡, Hamster WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-phosphoenolpyruvate Carboxykinase 1 (Soluble) (PCK1) (AA 311-558) antibodyPCK1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-phosphoenolpyruvate Carboxykinase 1 (Soluble) (PCK1) (AA 1-622) antibodyPCK1 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
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应用备注
- 1 : 500 (IL), 1: 1000 - : 10 000 (WB)
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- For reconstitution add 50 μL of sterile water
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缓冲液
- Immunogen affinity purified serum in PBS pH 7.4.
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注意事项
- Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube
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储存条件
- -20 °C
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储存方法
- Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Please do not re-use this primary antibody solution. In case of cyanobacterial samples there will be no signal in your second incubation.
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: "Conversion of carbon dioxide to oxaloacetate using integrated carbonic anhydrase and phosphoenolpyruvate carboxylase." in: Bioprocess and biosystems engineering, (2013) (PubMed).
: "The physiology of ex vitro pineapple (Ananas comosus L. Merr. var MD-2) as CAM or C3 is regulated by the environmental conditions: proteomic and transcriptomic profiles." in: Plant cell reports, (2013) (PubMed).
: "Ectopic expression of Rubisco subunits in maize mesophyll cells does not overcome barriers to cell type-specific accumulation." in: Plant physiology, Vol. 160, Issue 1, pp. 419-32, (2012) (PubMed).
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: "Conversion of carbon dioxide to oxaloacetate using integrated carbonic anhydrase and phosphoenolpyruvate carboxylase." in: Bioprocess and biosystems engineering, (2013) (PubMed).
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- PCK1 (phosphoenolpyruvate Carboxykinase 1 (Soluble) (PCK1))
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别名
- Phosphoenolpyruvate Carboxylase
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背景
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Background: PEPC (phosphoenolpyruvate carboxylase), EC=4.1.1.31, belongs to an enzyme family of carboxy-lyases that is catalyzing adding fo carbon dioxide to phosphoenolpyruvate (PEP) to form oxaloacetate. Alternative names: PEPCase 1, PEPCase 3, PEPC 1, PEPC 3
Additional information: PEPC is especially prone to proteolysis. To avoid artifacts, we use chymotrypsin, as recommended by Plaxton (2019), in addition to the Roche protease inhibitor cocktail.
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分子量
- 110 | 105 kDa
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基因ID
- 841765
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NCBI登录号
- NP_177043
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UniProt
- Q9MAH0, Q84VW9
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途径
- Positive Regulation of Peptide Hormone Secretion, Carbohydrate Homeostasis
抗原
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