RPS6KA1 抗体 (pSer732)
Quick Overview for RPS6KA1 抗体 (pSer732) (ABIN349634)
抗原
See all RPS6KA1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- pSer732
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Supplier Product No.
- 600-401-b30
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Supplier
- Rockland
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原理
- p90 RSK1 phospho S732 Antibody
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交叉反应 （详细）
- This antibody is specific for human p90 RSK1 protein phosphorylated at S732.
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产品特性
- Synonyms: rabbit anti-p90 RSK1 pS732 antibody, rabbit anti-RSK1 pS732 antibody, Ribosomal S6 Kinase 1, RSK-1, S6K-alpha 1, 90 kDa Ribosomal Protein S6 Kinase 1, MAP kinase-activated protein kinase 1a, MAPK-activated protein kinase 1a, p90-RSK 1, p90S6K, MAPKAP kinase 1a, MAPKAPK-1a, Ribosomal S6 kinase 1, RSK 1, RPS6KA1, MAPKAPK1A
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纯化方法
- This product was affinity purified from monospecific antiserum by immunoaffinity chromatography using phosphorylated peptide coupled to agarose beads followed by solid phase adsorption against non phosphorylated peptide.
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过滤
- Sterile filtered
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免疫原
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Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the C-terminal end of human RSK1 protein.
Immunogen Type: Conjugated Peptide
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亚型
- IgG
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anti-Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 1 (RPS6KA1) (AA 62-321) antibody
RPS6KA1 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 1 (RPS6KA1) (AA 1-735) antibodyRPS6KA1 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 4E11 unconjugated
anti-Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 1 (RPS6KA1) (C-Term) antibodyRPS6KA1 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 1 (RPS6KA1) (Thr573) antibodyRPS6KA1 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 1 (RPS6KA1) (pSer363), (pThr359) antibodyRPS6KA1 适用: 人 WB, ELISA, IP, IF 宿主: 兔 Monoclonal 1B6 unconjugated Recombinant Antibody
anti-Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 1 (RPS6KA1) (pSer363), (pThr359) antibodyRPS6KA1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 1 (RPS6KA1) (AA 539-588) antibodyRPS6KA1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 1 (RPS6KA1) (pSer380) antibodyRPS6KA1 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 1 (RPS6KA1) antibodyRPS6KA1 适用: 人 ELISA, IHC 宿主: 小鼠 Monoclonal 10B1D7A9 unconjugated
anti-Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 1 (RPS6KA1) (pThr348) antibodyRPS6KA1 适用: 人, 小鼠, 大鼠 WB, ELISA, IF (p), IF (cc), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
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应用备注
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Application Note: This affinity-purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. By western blot a band approximately 90 kDa in size corresponding to p90 RSK1 pS732 protein is expected in the appropriate cell lysate or extract.
Western Blot Dilution: 1:1000-1:3000
ELISA Dilution: 1:5000-1:50,000
Other: User Optimized
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1.7 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- RPS6KA1 (Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 1 (RPS6KA1))
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别名
- RPS6KA1
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背景
- Background: Ribosomal S6 Kinase 1 (RSK1, S6K-alpha 1, 90 kDa Ribosomal Protein S6 Kinase 1, MAP kinase-activated protein kinase 1a, or MAPKAPK1A) is an immediate downstream effector of mitogen activated protein kinases and therefore promotes cell proliferation and survival. It has serine/threonine kinase activity and may play a role in mediating the growth-factor and stress-induced activation of the transcription factor CREB. The C-terminal region of RSK1 is reported to be an ERK docking site, where serine 732 phosphorylation status is critical for RSK1 activation. When serine 732 is not phosphorylated, ERK1/2 binds to the ERK docking site of RSK1, and upon stimulation, activates RSK1. The activated RSK1 then autophosphorylates serine 732, leading to the dissociation of ERK from RSK1 and termination of activation by ERK. RSK1 is studied in cancer research and is known to inactive tumor suppressor complexes and death kinases.
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基因ID
- 6195
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NCBI登录号
- NP_001006666
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UniProt
- Q15418
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途径
- MAPK Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades
抗原
-
