OCT4 抗体 (Internal Region)
Quick Overview for OCT4 抗体 (Internal Region) (ABIN349596)
抗原
See all OCT4 (POU5F1) 抗体适用
宿主
克隆类型
标记
应用范围
-
-
抗原表位
- Internal Region
-
Supplier Product No.
- 600-401-a95
-
Supplier
- Rockland
-
原理
- Oct-4 Antibody
-
交叉反应 （详细）
- This antibody reacts with human and mouse Oct-4 protein.
-
产品特性
- Synonyms: rabbit anti-OCT4 antibody, OCT-4, OCT 4, Octamer binding protein 4 antibody, Octamer binding transcription factor 3 antibody, OTF3 antibody, OCT3, POU5F1, POU class 5 homeobox 1 antibody, POU domain class 5 transcription factor 1 antibody
-
纯化方法
- This product was affinity purified from monospecific antiserum by immunoaffinity chromatography.
-
过滤
- Sterile filtered
-
免疫原
-
Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human Oct-4 protein.
Immunogen Type: Conjugated Peptide
-
亚型
- IgG
-
-
anti-POU Class 5 Homeobox 1 (POU5F1) (AA 81-164) antibody
POU5F1 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 1B11 unconjugated
anti-POU Class 5 Homeobox 1 (POU5F1) antibodyKO Validated POU5F1 适用: 人 WB, IF, ICC, IP, FACS, IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-POU Class 5 Homeobox 1 (POU5F1) (AA 201-300) antibodyPOU5F1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-POU Class 5 Homeobox 1 (POU5F1) (AA 1-360) antibodyPOU5F1 适用: 人 WB, ELISA, IHC, IF, FACS 宿主: 小鼠 Monoclonal 2H41D8 unconjugated
anti-POU Class 5 Homeobox 1 (POU5F1) antibodyPOU5F1 适用: 人 WB, ELISA, ICC, FACS 宿主: 小鼠 Monoclonal 7E7 unconjugated
anti-POU Class 5 Homeobox 1 (POU5F1) (AA 136-360) antibodyPOU5F1 适用: 人 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-POU Class 5 Homeobox 1 (POU5F1) (AA 1-360) antibodyPOU5F1 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-POU Class 5 Homeobox 1 (POU5F1) (AA 81-164) antibodyPOU5F1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1D2 unconjugated
anti-POU Class 5 Homeobox 1 (POU5F1) (Internal Region) antibodyPOU5F1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-POU Class 5 Homeobox 1 (POU5F1) antibodyPOU5F1 适用: 人, 猴 WB, IF, IHC (p) 宿主: 兔 Polyclonal unconjugated
-
-
应用备注
-
Application Note: This affinity purified antibody has been tested for use in ELISA and western blotting.
Western Blot Dilution: 1:1,000 - 1:1,500
Immunoprecipitation Dilution: User Optimized
ELISA Dilution: 1:350,000
IF Microscopy Dilution: User Optimized
-
限制
- 仅限研究用
-
-
-
状态
- Liquid
-
浓度
- 1.0 mg/mL
-
缓冲液
-
Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
-
注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
储存条件
- 4 °C,-20 °C
-
储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
-
有效期
- 12 months
-
-
- OCT4 (POU5F1) (POU Class 5 Homeobox 1 (POU5F1))
-
别名
- POU5F1
-
背景
- Background: Oct-4, also known as POU domain transcription factor, Octomer- binding transcription factor 3 and Oct-3, is expressed in human and mouse totipotent embryonic stem and germ cells. It may function in preventing these cells from differentiating. Each type of established mammalian pluripotent stem cell line (embryonal carcinoma (EC) cells, embryonic stem (ES) cells and embryonic germ (EG) cells) expresses Oct-4, which disappears rapidly when the cells differentiate. Most studies on Oct-4 have been performed on murine embryos. In human oocytes of various maturity and normally developing preimplantation/cleavage embryos, a variable Oct-4 expression pattern has been revealed, concomitant with a pure cytoplasmic localization of the protein. This variability in expression fades away during compaction, indicating embryonic Oct-4 expression, the protein appears in the nucleus, implying biological activity. Functional Oct-4 has been found in human amniotic fluid. As Oct-4 transcripts have also been found in various differentiated cells, the presence of Oct-4 transcripts or proteins may not be sufficient for identifying undifferentiated cell lines in humans. It may also be important to examine the localization of Oct-4 proteins within a cell. Oct-4 has been shown to interact with Nanog and with proteins from multiple repression complexes, including the NuRD, Sin3A and Pml complexes, possibly to control ES fate. Reactivation of Oct-4 expression is postulated to occur in differentiated cells that have undergone carcinogenesis, or tumor formation.
-
基因ID
- 5460, 116235491
-
UniProt
- Q01860
-
途径
- Stem Cell Maintenance
抗原
-
