PKC beta 抗体 (Internal Region)
Quick Overview for PKC beta 抗体 (Internal Region) (ABIN349591)
抗原
See all PKC beta (PRKCB) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- Internal Region
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Supplier Product No.
- 600-401-a76
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Supplier
- Rockland
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原理
- Protein Kinase C Beta Antibody
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交叉反应 （详细）
- This antibody is specific for human PKC beta protein.
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产品特性
- Synonyms: rabbit anti-Protein kinase C beta antibody, PKC ß, Protein kinase C beta type, PRKCB, PKCB, PRKCB1, PKC-beta, PKC-B
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纯化方法
- This product was affinity purified from monospecific antiserum by immunoaffinity chromatography.
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过滤
- Sterile filtered
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免疫原
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Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human Protein kinase C beta.
Immunogen Type: Conjugated Peptide
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亚型
- IgG
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anti-Protein Kinase C, beta (PRKCB) (AA 1-270) antibody
PRKCB 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Protein Kinase C, beta (PRKCB) (AA 220-340) antibodyPRKCB 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Protein Kinase C, beta (PRKCB) (pThr500) antibodyPRKCB 适用: 人, 大鼠 WB, ELISA, FACS, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Protein Kinase C, beta (PRKCB) (AA 517-643) antibodyPRKCB 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Protein Kinase C, beta (PRKCB) (pThr641) antibodyPRKCB 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Protein Kinase C, beta (PRKCB) (Internal Region) antibodyVerified PRKCB 适用: 人, 大鼠 WB, ELISA, IHC 宿主: 山羊 Polyclonal unconjugated
anti-Protein Kinase C, beta (PRKCB) (AA 642-673) antibodyPRKCB 适用: 人, 大鼠, 小鼠, 灵长类 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Protein Kinase C, beta (PRKCB) (AA 601-673) antibodyPRKCB 适用: 人, 大鼠, 小鼠 WB, ELISA, FACS, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Protein Kinase C, beta (PRKCB) (AA 261-341) antibodyPRKCB 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-Protein Kinase C, beta (PRKCB) (AA 1-671) antibodyPRKCB 适用: 人 WB, PLA 宿主: 兔 Polyclonal unconjugated
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应用备注
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Application Note: This affinity purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 76 kDa in size corresponding to PKC beta by western blotting in the appropriate cell lysate or extract. Lysates from U251 or BxPC3 cells are suggested for western blotting.
Western Blot Dilution: 1:1,000 - 1:2,000
ELISA Dilution: 1:75,000 - 1:80,000
Other: User Optimized
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1.0 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- PKC beta (PRKCB) (Protein Kinase C, beta (PRKCB))
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别名
- PRKCB
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背景
- Background: This antibody is designed, produced, and validated as part of a collaboration with the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. Protein kinase C (PKC) beta is implicated as a potential important factor in cancer cell proliferation and angiogenesis. PKC is a family of serine- and threonine-specific protein kinases that can be activated by calcium and second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This protein kinase has been reported to be involved in many different cellular functions, such as B cell activation, apoptosis induction, endothelial cell proliferation, and intestinal sugar absorption. Studies in mice also suggest that this kinase may also regulate neuronal functions and correlate fear-induced conflict behavior after stress. Alternatively spliced transcript variants encoding distinct isoforms have been reported.
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基因ID
- 5579, 47157322
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UniProt
- P05771
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途径
- WNT signaling, TCR Signaling, Thyroid Hormone Synthesis, Nuclear Hormone Receptor Binding, Chromatin Binding, Myometrial Relaxation and Contraction, VEGF Signaling, Unfolded Protein Response, BCR Signaling
抗原
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