COL2 抗体
Quick Overview for COL2 抗体 (ABIN344746)
抗原
See all COL2 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- Recognizes both alpha 1 (II) and alpha 3 (XI) chains which have identical primary structure. It also reacts with pepsin-digested type II collagen. Its epitope is localized in the triple helix of type II collagen. It shows no cross-reaction with type I or type III collagen. Species cross-reactivity: Human, Chicken, Mouse, Salamander.
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纯化方法
- Purified
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免疫原
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Purified type II collagen, obtained from adult chicken sternum by limited pepsinization.
Type of Immunogen: Purified protein -
亚型
- IgG1
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anti-Collagen, Type II (COL2) (AA 1201-1300) antibody
COL2 适用: 人, 小鼠, 大鼠, 兔, 豚鼠 WB, ELISA, IHC (p), IHC (fro), FACS, IF (cc), IF (p) 宿主: 兔 Polyclonal unconjugated
anti-Collagen, Type II (COL2) antibodyCOL2 适用: 人 ELISA 宿主: 山羊 Polyclonal unconjugated
anti-Collagen, Type II (COL2) (N-Term) antibodyCOL2 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF/ICC 宿主: 兔 Polyclonal unconjugated
anti-Collagen, Type II (COL2) (AA 1401-1487) antibodyCOL2 适用: 人, 大鼠, 兔 WB, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Collagen, Type II (COL2) (AA 101-150) antibodyCOL2 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Collagen, Type II (COL2) antibody (Biotin)COL2 适用: 人 ELISA 宿主: 山羊 Polyclonal Biotin
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应用备注
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Approved: ELISA, IHC, IHC-P (1:200), WB (1:200 - 1:400)
Usage: In Western blotting, antibody reacts with the TCA fragment of lathyritic type II collagen after digestion with mammalian collagenase. Western Blot of CNBr peptides of collagen II shows that this antibody reacts with CB11 fragment which is the site of immunogenic and arthritogenic epitopes along the intact type II molecule. ELISA. Western Blot: 1:200-1:400. Cartilage is an appropriate positive control. Immunohistochemistry (Formalin/Paraffin): 1-2 μg/mL. Staining of paraffin embedded tissues requires digestion of tissue sections with pepsin at 1 mg/mL Tris HCl, pH 2 for 15 minutes at RT or 15 minutes at 37?C. -
说明
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Target Species of Antibody: Chicken
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- Lot specific
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缓冲液
- 10 mM PBS, pH 7.4, 0.2 % BSA, 15 mM sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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- COL2 (Collagen, Type II (COL2))
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别名
- Collagen Type II
抗原
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