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CD4 抗体

This anti-CD4 antibody is a 大鼠 单克隆 antibody detecting CD4 in FACS, IP, IHC (fro), ICC 和 Func. Suitable for 小鼠. This Primary Antibody has been cited in 9+ publications.
Pubmed (9 references)
产品编号 ABIN343704
发货至: 中国

Quick Overview for CD4 抗体 (ABIN343704)

抗原

See all CD4 抗体
CD4

适用

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小鼠

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大鼠

克隆类型

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单克隆

标记

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This CD4 antibody is un-conjugated

应用范围

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Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC), Functional Studies (Func)

克隆位点

GK1-5
Discover our top product CD4 Primary Antibody

  • 原理

    Anti-Ms CD4 Purified Azide Free

    特异性

    The rat monoclonal antibody GK1.5 reacts with an extracellular epitope of mouse CD4 transmembrane glycoprotein (55 kDa).

    交叉反应 （详细）

    Mouse

    纯化方法

    Purified by protein-G affinity chromatography.

    纯度

    > 95 % (by SDS-PAGE)

    免疫原

    Mouse CTL clone V4 cells

    亚型

    IgG2b
  • anti-CD4 (CD4) (AA 385-457) antibody
    anti-CD4 (CD4) (AA 385-457) antibody

    CD4 适用: 人, 小鼠, 大鼠, 小鸡, Pig FACS, WB, ELISA, IHC (fro), IHC (p), IF (cc), IF (p) 宿主: 兔 Polyclonal unconjugated

    anti-CD4 (CD4) antibody
    anti-CD4 (CD4) antibody

    CD4 适用: 人, 小鼠, 大鼠, 小鸡, 猴, 犬, Pig, 兔, Cat, Cow, 绵羊, 豚鼠, 山羊, 马, Avian, 驴, Hamster WB, IHC 宿主: 山羊 Polyclonal unconjugated

    anti-CD4 (CD4) (AA 231-330) antibody
    anti-CD4 (CD4) (AA 231-330) antibody

    CD4 适用: 人, 小鼠, 大鼠 FACS, WB, ELISA, IHC (fro), IHC (p), IF (cc), IF (p) 宿主: 兔 Polyclonal unconjugated

    anti-CD4 (CD4) (N-Term) antibody
    anti-CD4 (CD4) (N-Term) antibody

    CD4 适用: 人 FACS, WB, MS 宿主: 小鼠 Monoclonal MEM-241 unconjugated

    anti-CD4 (CD4) (AA 301-400) antibody
    anti-CD4 (CD4) (AA 301-400) antibody

    CD4 适用: 人, 小鼠, 大鼠 FACS, WB, IHC (p) 宿主: 兔 Polyclonal unconjugated

    anti-CD4 (CD4) (N-Term) antibody (FITC)
    anti-CD4 (CD4) (N-Term) antibody (FITC)

    CD4 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-241 FITC

    anti-CD4 (CD4) antibody (PE)
    anti-CD4 (CD4) antibody (PE)

    CD4 适用: 大鼠 FACS 宿主: 小鼠 Monoclonal OX-35 PE

    anti-CD4 (CD4) (N-Term) antibody (PE)
    anti-CD4 (CD4) (N-Term) antibody (PE)

    CD4 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-241 PE

    anti-CD4 (CD4) (AA 26-200) antibody
    anti-CD4 (CD4) (AA 26-200) antibody

    CD4 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated

    anti-CD4 (CD4) (AA 58-86) antibody
    anti-CD4 (CD4) (AA 58-86) antibody

    CD4 适用: 人 FACS, WB, ELISA 宿主: 兔 Polyclonal unconjugated

  • 应用备注

    Functional application: Isolation and depletion of CD4+ T cells, blocking of ligand binding to CD4.
    Immunocytochemistry: Recommended dilution: 1-4 μg/mL.
    Immunoprecipitation: Recommended dilution: 1-2 μg / 100-500 μg of protein in 1 mL lysate.
    Flow cytometry: Recommended dilution: 1 μg/million cells.
    Immunohistochemistry: Recommended dilution: 5-10 μg/mL.

    限制

    仅限研究用

  • 浓度

    1 mg/mL

    缓冲液

    Phosphate buffered saline (PBS), pH 7.4

    储存液

    Azide free

    注意事项

    Do not freeze.

    储存条件

    4 °C

    储存方法

    Store at 2-8°C. Do not freeze.
  • Hu, Watson, Zhang, Graf, Wang, Sartor, Howden, Fletcher, Alexander: "Long-term cardiac allograft survival across an MHC mismatch after "pruning" of alloreactive CD4 T cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 180, Issue 10, pp. 6593-603, (2008) (PubMed).

    Yi, Zhen, Zeng, Zhang, Zhao: "Depleting anti-CD4 monoclonal antibody (GK1.5) treatment: influence on regulatory CD4+CD25+Foxp3+ T cells in mice." in: Transplantation, Vol. 85, Issue 8, pp. 1167-74, (2008) (PubMed).

    Felix, Donermeyer, Horvath, Walters, Gross, Suri, Allen: "Alloreactive T cells respond specifically to multiple distinct peptide-MHC complexes." in: Nature immunology, Vol. 8, Issue 4, pp. 388-97, (2007) (PubMed).

    Zheng, Han, Kelsoe: "T helper cells in murine germinal centers are antigen-specific emigrants that downregulate Thy-1." in: The Journal of experimental medicine, Vol. 184, Issue 3, pp. 1083-91, (1997) (PubMed).

    Gavett, Chen, Finkelman, Wills-Karp: "Depletion of murine CD4+ T lymphocytes prevents antigen-induced airway hyperreactivity and pulmonary eosinophilia." in: American journal of respiratory cell and molecular biology, Vol. 10, Issue 6, pp. 587-93, (1994) (PubMed).

    Godfrey, Kennedy, Gately, Hakimi, Hubbard, Zlotnik: "IL-12 influences intrathymic T cell development." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 152, Issue 6, pp. 2729-35, (1994) (PubMed).

    Wu, Antica, Johnson, Scollay, Shortman: "Developmental potential of the earliest precursor cells from the adult mouse thymus." in: The Journal of experimental medicine, Vol. 174, Issue 6, pp. 1617-27, (1992) (PubMed).

    Dialynas, Quan, Wall, Pierres, Quintáns, Loken, Pierres, Fitch: "Characterization of the murine T cell surface molecule, designated L3T4, identified by monoclonal antibody GK1.5: similarity of L3T4 to the human Leu-3/T4 molecule." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 131, Issue 5, pp. 2445-51, (1983) (PubMed).

    Dialynas, Wilde, Marrack, Pierres, Wall, Havran, Otten, Loken, Pierres, Kappler et al.: "Characterization of the murine antigenic determinant, designated L3T4a, recognized by monoclonal antibody GK1.5: expression of L3T4a by functional T cell clones appears to correlate primarily with ..." in: Immunological reviews, Vol. 74, pp. 29-56, (1983) (PubMed).

  • 抗原

    CD4

    别名

    CD4

    背景

    CD4 Molecule,CD4 (T4) is a single chain transmembrane glycoprotein and belongs to immunoglobulin supergene family. In extracellular region there are 4 immunoglobulin-like domains (1 Ig-like V-type and 3 Ig-like C2-type). Transmembrane region forms 25 aa, cytoplasmic tail consists of 38 aa. Domains 1,2 and 4 are stabilized by disulfide bonds. The intracellular domain of CD4 is associated with p56Lck, a Src-like protein tyrosine kinase. It was described that CD4 segregates into specific detergent-resistant T-cell membrane microdomains. Extracellular ligands: MHC class II molecules (binds to CDR2-like region in CD4 domain 1), HIV envelope protein gp120 (binds to CDR2-like region in CD4 domain 1), IL-16 (binds to CD4 domain 3), human seminal plasma glycoprotein gp17 (binds to CD4 domain 1), L-selectin. Intracellular ligands: p56LckCD4 is a co-receptor involved in immune response (co-receptor activity in binding to MHC class II molecules) and HIV infection (human immunodeficiency virus, CD4 is primary receptor for HIV-1 surface glycoprotein gp120). CD4 regulates T-cell activation, T/B-cell adhesion, T-cell diferentiation, T-cell selection and signal transduction. Defects in antigen presentation (MHC class II) cause dysfunction of CD4+ T-cells and their almost complete absence in patients blood, tissue and organs (SCID immunodeficiency).,T4/Leu-3, L3T4

    基因ID

    12504

    UniProt

    P06332

    途径

    TCR Signaling, Maintenance of Protein Location, CXCR4-mediated Signaling Events
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