CD34 抗体
(6 references)Quick Overview for CD34 抗体 (ABIN343702)
抗原
See all CD34 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Anti-Hu CD34 Purified Azide Free
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特异性
- The antibody QBEnd-10 reacts with an extracellular class II epitope on CD34, a 110-115 kDa monomeric transmembrane phosphoglycoprotein expressed on hematopoietic progenitors cells and on the most pluripotential stem cells, it is gradually lost on progenitor cells. This antibody has been also used as an endothelial marker.
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无交叉反应
- Cow, 犬, 大鼠, 绵羊
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交叉反应 （详细）
- Human, Non-Human Primates
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纯化方法
- Purified by protein-A affinity chromatography.
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纯度
- > 95 % (by SDS-PAGE)
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免疫原
- Human endothelial vesicles
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亚型
- IgG1
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anti-CD34 (CD34) (AA 366-382), (C-Term) antibody
CD34 适用: 人, 大鼠, 小鼠 FACS, WB, IHC, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-CD34 (CD34) (AA 201-300) antibodyCD34 适用: 人, 大鼠, 小鼠, 兔 FACS, WB, ELISA, IHC (p), IHC (fro), IF (p) 宿主: 兔 Polyclonal unconjugated
anti-CD34 (CD34) (AA 301-385) antibodyCD34 适用: 人, 大鼠, 小鼠, 兔, 小鸡, 山羊 FACS, WB, ELISA, IHC (p), IHC (fro), IF (p), IF (cc) 宿主: 兔 Polyclonal unconjugated
anti-CD34 (CD34) (AA 36-287) antibodyCD34 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-CD34 (CD34) antibodyCD34 适用: 人, 大鼠 FACS, IHC, StM 宿主: 兔 Monoclonal HPCA1-2598R unconjugated Recombinant Antibody
anti-CD34 (CD34) antibody (PE)CD34 适用: 人 FACS, ICC 宿主: 小鼠 Monoclonal 4H11[APG] PE
anti-CD34 (CD34) (C-Term) antibodyCD34 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-CD34 (CD34) (AA 151-385) antibodyCD34 适用: 人, 大鼠 WB, IHC, IF, ELISA 宿主: 兔 Polyclonal unconjugated
anti-CD34 (CD34) (AA 124-375) antibodyCD34 适用: 人 WB, IHC, IF, ELISA 宿主: 兔 Polyclonal unconjugated
anti-CD34 (CD34) antibodyCD34 适用: 人 FACS, WB, IHC (p), ICC 宿主: 小鼠 Monoclonal 4H11[APG] unconjugated
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应用备注
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Functional application: The antibody QBEnd-10 induces homotypic adhesion of leukemic cell line.
Flow cytometry: Recommended dilution: 5 μg/mL.
Immunohistochemistry (paraffin sections): Recommended dilution: 2-8 μg/mL.
Western blotting: Recommended dilution: 1-2 μg/mL, positive control: TF-1 cells. -
限制
- 仅限研究用
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浓度
- 1 mg/mL
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缓冲液
- Phosphate buffered saline (PBS), pH 7.4
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储存液
- Azide free
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注意事项
- Do not freeze.
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储存条件
- 4 °C
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储存方法
- Store at 2-8°C. Do not freeze.
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: "QBEND/10 (anti-CD34 antibody) in external root sheath cells and follicular tumors." in: Journal of cutaneous pathology, Vol. 21, Issue 3, pp. 224-8, (1994) (PubMed).
: "Certain anti-CD34 monoclonal antibodies induce homotypic adhesion of leukemic cell lines in a CD18-dependent and a CD18-independent way." in: European journal of immunology, Vol. 24, Issue 10, pp. 2304-11, (1994) (PubMed).
: "Heterogeneity of vascular endothelial cells with relevance to diagnosis of vascular tumours." in: Journal of clinical pathology, Vol. 45, Issue 2, pp. 143-8, (1992) (PubMed).
: "Differential sensitivity of CD34 epitopes to cleavage by Pasteurella haemolytica glycoprotease: implications for purification of CD34-positive progenitor cells." in: Experimental hematology, Vol. 20, Issue 5, pp. 590-9, (1992) (PubMed).
: "QBEND/10, a new monoclonal antibody to endothelium: assessment of its diagnostic utility in paraffin sections." in: Histopathology, Vol. 17, Issue 3, pp. 237-42, (1991) (PubMed).
: "Leukocyte antigen CD34 is expressed by a subset of cultured endothelial cells and on endothelial abluminal microprocesses in the tumor stroma." in: Laboratory investigation; a journal of technical methods and pathology, Vol. 62, Issue 6, pp. 690-6, (1990) (PubMed).
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: "QBEND/10 (anti-CD34 antibody) in external root sheath cells and follicular tumors." in: Journal of cutaneous pathology, Vol. 21, Issue 3, pp. 224-8, (1994) (PubMed).
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- CD34
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别名
- CD34
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背景
- CD34 Molecule,CD34 is a highly glycosylated monomeric 111-115 kDa surface protein, which is present on many stem cell populations. It is a well established stem cell marker, though its expression on human hematopoietic stem cells is reversible. CD34 probably serves as a surface receptor that undergoes receptor-mediated endocytosis and regulates adhesion, differentiation and proliferation of hematopoietic stem cells and other progenitors. CD34 expression is likely to represent a specific state of hematopoietic development that may have altered adhering properties with expanding and differentiating capabilities in both in vitro and in vivo conditions.
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基因ID
- 947
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UniProt
- P28906
抗原
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