Immunoprecipitation experiments were performed testing antibody concentrations ranging from 16 to 0.08 μg/mL and assayed by A405 nm spectrophotometry. Nearly complete immunoprecipitation was obtained at 8 μg/mL. The control was non-immune immunoglobulin.
纯化方法
Protein G Column
免疫原
Immunogen was brominated poly-(DG-DC)-left handed DNA (Z-DNA) complexed to methylated BSA.