Nitrotyrosine 抗体
Quick Overview for Nitrotyrosine 抗体 (ABIN339840)
抗原
See all Nitrotyrosine 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
 - Detects proteins and peptides containing nitro-tyrosine in a manner independent of the surrounding amino acid sequence. It is a valuable tool for identifying new nitrated proteins as well as for assaying protein nitration and measuring levels of nitrated proteins in tissues and samples. Does not cross-react with unmodified tyrosine or with phospho-tyrosine.
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纯化方法
 - Protein A purified
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免疫原
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                        Synthetic nitro-tyrosine containing peptides coupled to KLH.
Type of Immunogen: Synthetic peptide - 
                                            
亚型
 - IgG
 
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应用备注
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                        Approved: ELISA (1:1000), ICC, WB (1:1000)
Usage: Suitable for use in ELISA, Western Blot and Immunocytochemistry (ABC detection method). Western Blot: 1:1000, incubate membrane with diluted antibody in 5 % BSA, 1X TBS, 0.1 % Tween-20 at 4?C with gentle shaking, overnight. Immunocytochemistry (ABC): 1:50. ELISA: 1:1000. - 
                                            
限制
 - 仅限研究用
 
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状态
 - Liquid
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浓度
 - Lot specific
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缓冲液
 - 10 mM HEPES, pH 7.5, 150 mM sodium chloride, 0.1 mg/mL BSA, 50 % glycerol. No preservative added.
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储存液
 - Without preservative
 
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- Nitrotyrosine
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物质类
 - Chemical
 
抗原
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