HSF1 抗体
Quick Overview for HSF1 抗体 (ABIN337399)
抗原
See all HSF1 抗体适用
宿主
克隆类型
标记
应用范围
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品牌
- IHC-plus™
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特异性
- Recognizes human HSF-1 at ~85kD protein in unstressed cells and an ~95kD protein in heat shocked cells, corresponding to the molecular masses of the inactive and activated forms respectively of HSF-1. Species cross-reactivity: mouse, rat, monkey, rabbit.
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纯化方法
- Protein A purified
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免疫原
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Recombinant human HSF-1 expressed in E. coli.
Type of Immunogen: Recombinant protein -
亚型
- IgG
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应用备注
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Approved: IHC, IHC-P (5 μg/mL), WB (1:500)
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Positive control: Recombinant Human HSF-1 Protein. -
说明
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Target Species of Antibody: Human
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- Lot specific
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缓冲液
- PBS, 0.09 % sodium azide, 50 % glycerol.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C,-20 °C
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储存方法
- 4°C or -20°C, Avoid freeze-thaw cycles.
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- HSF1 (Heat Shock Factor Protein 1 (HSF1))
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别名
- HSF1
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背景
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Name/Gene ID: HSF1
Synonyms: HSF1, HSTF 1, Heat shock factor protein 1, HSF 1, HSTF1 -
基因ID
- 3297
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UniProt
- Q00613
抗原
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