Caspase 6 抗体 (Cleavage Site)
Quick Overview for Caspase 6 抗体 (Cleavage Site) (ABIN337396)
抗原
See all Caspase 6 (CASP6) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- Cleavage Site
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品牌
- IHC-plus™
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特异性
- Caspase-6 Antibody recognizes endogenous levels of both full length caspase-6 (35kD) and the small subunit of caspase-6 resulting from cleavage at Asp194 (15kD). This antibody does not recognize other caspases. Recognizes human, mouse, rabbit.
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纯化方法
- Protein A purified
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免疫原
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Synthetic peptide KLH coupled corresponding to residues surrounding the cleavage site of caspase-6.
Type of Immunogen: Synthetic peptide -
亚型
- IgG
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应用备注
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Approved: ELISA, IHC, IHC-P (5 μg/mL), WB (1:1000)
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. -
说明
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Target Species of Antibody: Mouse
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- Lot specific
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缓冲液
- 10 mM HEPES, pH 7.5, 150 mM sodium chloride, 0.1 mg/mL BSA, 50 % glycerol. No preservative added.
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储存液
- Without preservative
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C,-20 °C
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储存方法
- 4°C or -20°C, Avoid freeze-thaw cycles.
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- Caspase 6 (CASP6) (Caspase 6, Apoptosis-Related Cysteine Peptidase (CASP6))
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别名
- CASP6 / Caspase 6
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背景
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Name/Gene ID: CASP6
Subfamily: Cysteine C14
Family: Protease
Synonyms: CASP6, Apoptotic protease Mch-2, Caspase 6, CASP-6, Caspase-6, Mch-2, MCH2 -
基因ID
- 839
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UniProt
- P55212
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途径
- Apoptosis, Caspase Cascade in Apoptosis
抗原
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