DUSP1 抗体 (C-Term)
Quick Overview for DUSP1 抗体 (C-Term) (ABIN337195)
抗原
See all DUSP1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 87-101, C-Term
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品牌
- IHC-plus™
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特异性
- Recognizes human MKP1, Mr ~42kD. Species cross-reactivity: mouse. Predicted to cross-react with rat based on sequence homology.
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预测反应
- Percent identity by BLAST analysis: Human, Gorilla, Marmoset, Rat, Dog (100%) Monkey, Mouse, Panda, Bovine, Rabbit (93%) Hamster, Pig (87%) Elephant (80%).
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纯化方法
- Protein A purified
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免疫原
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KLH-conjugated, synthetic peptide corresponding to amino acids 87-101 (DERSAALDGAKRDGT-C) of human MAP kinase phosphatase 1 (MKP1), with a C-terminal cysteine added for conjugation purposes. The immunizing sequence is identical in rat and mouse. Percent identity by BLAST analysis: Human, Gorilla, Marmoset, Rat, Dog (100%), Monkey, Mouse, Panda, Bovine, Rabbit (93%), Hamster, Pig (87%), Elephant (80%).
Type of Immunogen: Synthetic peptide - KLH conjugated -
亚型
- IgG
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anti-Dual Specificity Phosphatase 1 (DUSP1) (C-Term) antibody
Verified DUSP1 适用: 人 WB, ELISA, IHC 宿主: 山羊 Polyclonal unconjugated
anti-Dual Specificity Phosphatase 1 (DUSP1) (AA 151-250) antibodyDUSP1 适用: 人, 小鼠, 大鼠 WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Dual Specificity Phosphatase 1 (DUSP1) (AA 305-367) antibodyDUSP1 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 3A9 unconjugated
anti-Dual Specificity Phosphatase 1 (DUSP1) (AA 305-367) antibodyDUSP1 适用: 人 WB, ELISA, PLA 宿主: 小鼠 Monoclonal 4H7 unconjugated
anti-Dual Specificity Phosphatase 1 (DUSP1) (C-Term) antibodyDUSP1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Dual Specificity Phosphatase 1 (DUSP1) (AA 1-300) antibodyDUSP1 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Dual Specificity Phosphatase 1 (DUSP1) (Ser31) antibodyDUSP1 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Dual Specificity Phosphatase 1 (DUSP1) (C-Term) antibodyDUSP1 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Dual Specificity Phosphatase 1 (DUSP1) (AA 125-236) antibodyDUSP1 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
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应用备注
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Approved: IHC, IHC-P (5 μg/mL), WB (0.1 - 1 μg/mL)
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: 0.1-1 μg/mL detects MKP1 in RIPA lysates from 3T3/A31 cells. 3T3/A31 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-MKP1 (0.3 μg/mL). -
说明
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Target Species of Antibody: Human
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- Lot specific
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缓冲液
- 0.1 M Tris-glycine, pH 7.4, 0.15 M sodium chloride, 0.05 % sodium azide, before the addition of glycerol to 30 %
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C,-20 °C
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储存方法
- Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
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- DUSP1 (Dual Specificity Phosphatase 1 (DUSP1))
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别名
- MKP-1 / DUSP1
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背景
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Name/Gene ID: DUSP1
Subfamily: Dual specificity MKP
Family: Protein Phosphatase
Synonyms: DUSP1, 3CH134, Dual specificity phosphatase 1, ERK-inactivating phosphatase, HVH1, MAP kinase phosphatase 1, VH1, PTPN10, CL100, MKP-1, MKP1 -
基因ID
- 1843
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UniProt
- P28562
抗原
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