PAX2A 抗体 (pSer393)
Quick Overview for PAX2A 抗体 (pSer393) (ABIN337176)
抗原
See all PAX2A 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- pSer393
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品牌
- IHC-plus™
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特异性
- Recognizes human Pax2 protein when phosphorylated at Ser393. Based on amino acid sequence homology with Pax5 and Pax8, cross-reactivity with these proteins is likely. Does not react with purified, unphosphorylated GST-Pax2 proteins. Reacts with human HEK293 cell lysates and purified GST-Pax2 proteins phosphorylated by JNK. Species sequence Homology: mouse, chicken, zebra sh and frog - 100 % .
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纯化方法
- Immunoaffinity purified
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免疫原
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Synthetic phospho-peptide derived from the sequence surrounding the phosphorylated Ser393 residue of mouse and human Pax2 (paired box protein 2).
Type of Immunogen: Synthetic phospho-peptide -
亚型
- IgG
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anti-Paired Box Gene 2a (PAX2A) (AA 194-303) antibody
PAX2A 适用: 人 WB, ELISA, IHC (p) 宿主: 小鼠 Monoclonal 3C7 unconjugated
anti-Paired Box Gene 2a (PAX2A) (AA 223-354) antibodyPAX2A 适用: 人 ELISA, IHC, Coat, StM 宿主: 小鼠 Monoclonal PAX2-1104 unconjugated
anti-Paired Box Gene 2a (PAX2A) antibodyPAX2A 适用: 人, 小鼠, 大鼠 IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Paired Box Gene 2a (PAX2A) (AA 194-304) antibodyPAX2A 适用: 人 ELISA, FACS 宿主: 小鼠 Monoclonal 2C8C6 unconjugated
anti-Paired Box Gene 2a (PAX2A) (AA 248-282), (C-Term) antibodyPAX2A 适用: 人, 小鼠, 大鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Paired Box Gene 2a (PAX2A) antibodyPAX2A 适用: 人, 小鼠, 大鼠, Cow, 犬, Pig, 小鸡 WB, FACS 宿主: 兔 Polyclonal unconjugated
anti-Paired Box Gene 2a (PAX2A) (AA 194-304) antibodyPAX2A 适用: 人 WB, ELISA, IHC, IHC (p) 宿主: 小鼠 Monoclonal 3C7 unconjugated
anti-Paired Box Gene 2a (PAX2A) (Middle Region) antibodyPAX2A 适用: 人, 小鼠, 大鼠, 斑马鱼, Cow, 犬, 兔, 绵羊, 豚鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Paired Box Gene 2a (PAX2A) (AA 1-431) antibodyPAX2A 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Paired Box Gene 2a (PAX2A) (AA 174-223) antibodyPAX2A 适用: 人, 斑马鱼, Cow, 犬, 兔, 绵羊, 豚鼠, 马, 猴, Pig, 小鸡, Bat WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
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应用备注
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Approved: ELISA (0.1 - 1 μg/mL), IHC, IHC-P (1:50), WB (1 - 3 μg/mL)
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 1:50. Western Blot: Analysis of JNK-stimulated (phosphorylated) fusion proteins identifies a single band at ~50kD. In Western Blot analysis of HEK293 cells, a thick band between ~42-45kD is observed, which may represent different human Pax2 isoforms and/or Pax8. -
说明
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Target Species of Antibody: Human
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- Lot specific
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缓冲液
- PBS, pH 7.4, 0.1 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C,-20 °C
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储存方法
- 4°C or -20°C, Avoid freeze-thaw cycles.
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- PAX2A (Paired Box Gene 2a (PAX2A))
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别名
- PAX2
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背景
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Name/Gene ID: PAX2
Synonyms: PAX2, Paired box homeotic gene 2, Paired box protein Pax-2, PAPRS, Paired box 2, Paired box gene 2 -
基因ID
- 5076
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UniProt
- Q02962
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途径
- Carbohydrate Homeostasis, Stem Cell Maintenance, Tube Formation
抗原
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