PRMT5 抗体
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北京 101111
Quick Overview for PRMT5 抗体 (ABIN337159)
抗原
See all PRMT5 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- Recognizes endogenous levels of total human Skb1Hs methyltransferase protein at 70kD. Does not cross-react with other related mammalian methyltransferases. Species cross-reactivity: monkey, mouse and rat.
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纯化方法
- Protein A purified
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免疫原
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Synthetic peptide (KLH coupled) corresponding to residues surrounding amino acid 100 of human Skb1Hs methyltransferase.
Type of Immunogen: Synthetic peptide -
亚型
- IgG
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应用备注
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Approved: IHC, IHC-P (1:100), IP (1:100), WB (1:1000)
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 1:100. -
说明
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Target Species of Antibody: Human
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- Lot specific
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缓冲液
- 10 mM HEPES, pH 7.5, 150 mM sodium chloride, 0.1 mg/mL BSA, 50 % glycerol. No preservative added.
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储存液
- Without preservative
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C,-20 °C
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储存方法
- 4°C or -20°C, Avoid freeze-thaw cycles.
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- PRMT5 (Protein Arginine Methyltransferase 5 (PRMT5))
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别名
- PRMT5
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背景
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Name/Gene ID: PRMT5
Synonyms: PRMT5, 72 kDa ICln-binding protein, HRMT1L5, JBP1, Jak-binding protein 1, IBP72, SKB1Hs, SKB1, SKB1 homolog, Skb1 (S. pombe) homolog, SKB1 homolog (S. pombe) -
基因ID
- 10419
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UniProt
- O14744
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途径
- Chromatin Binding, Regulation of Muscle Cell Differentiation, Ribonucleoprotein Complex Subunit Organization, Skeletal Muscle Fiber Development
抗原
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