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Osteopontin 抗体 (AA 288-299)

This 山羊 多克隆 antibody specifically detects Osteopontin in WB, IHC, ELISA 和 IHC (p). It exhibits reactivity toward 人.
产品编号 ABIN337138
发货至: 中国
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Quick Overview for Osteopontin 抗体 (AA 288-299) (ABIN337138)

抗原

See all Osteopontin (SPP1) 抗体
Osteopontin (SPP1) (Secreted phosphoprotein 1 (SPP1))

适用

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宿主

  • 134
  • 63
  • 4
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山羊

克隆类型

  • 122
  • 82
多克隆

标记

  • 104
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This Osteopontin antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
  • 抗原表位

    • 15
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    AA 288-299

    特异性

    Human SPP1 / Osteopontin.

    预测反应

    Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey (100%) Marmoset (92%) Mouse, Panda, Dog, Pig, Arthrobotrys (83%).

    纯化方法

    Immunoaffinity purified

    免疫原

    Synthetic peptide C-DPKSKEEDKHLK from the C-terminus of human SPP1 / Osteopontin (NP_001035147.1, NP_000573.1, NP_001035149.1). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey (100%), Marmoset (92%), Mouse, Panda, Dog, Pig, Arthrobotrys (83%).

    Type of Immunogen: Synthetic peptide
  • 应用备注

    Approved: IHC, IHC-P (5 μg/mL), Peptide-ELISA (1:64000), WB (1 - 3 μg/mL)

    Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

    说明

    Target Species of Antibody: Human

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    Lot specific

    缓冲液

    Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Minimize freezing and thawing.

    储存条件

    -20 °C

    储存方法

    Store at -20°C. Avoid freeze-thaw cycles.
  • 抗原

    Osteopontin (SPP1) (Secreted phosphoprotein 1 (SPP1))

    别名

    SPP1 / Osteopontin

    背景

    Name/Gene ID: SPP1

    Synonyms: SPP1, BSPI, BNSP, Bone sialoprotein 1, ETA-1, Nephropontin, OPN, Secreted phosphoprotein 1, SPP-1, SPP1/CALPHA1 fusion, Urinary stone protein, Uropontin, Osteopontin, Bone sialoprotein I

    基因ID

    6696

    NCBI登录号

    NP_001035147, NP_000573, NP_001035149

    UniProt

    P10451

    途径

    Regulation of Cell Size
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