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CTNNB1 抗体 (C-Term)

CTNNB1 适用: 人, 小鼠, 大鼠, 小鸡, 非洲爪蟾 WB, IHC, ELISA, IHC (p), IP 宿主: 兔 Polyclonal unconjugated
产品编号 ABIN337082
发货至: 中国
  • 抗原 See all CTNNB1 抗体
    CTNNB1 (Catenin (Cadherin-Associated Protein), beta 1, 88kDa (CTNNB1))
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    C-Term
    适用
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    人, 小鼠, 大鼠, 小鸡, 非洲爪蟾
    宿主
    • 153
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    克隆类型
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    多克隆
    标记
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    This CTNNB1 antibody is un-conjugated
    应用范围
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    Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)
    品牌
    IHC-plus™
    特异性
    Recognizes human b-catenin. Species cross-reactivity: chicken, mouse, rat and Xenopus.
    纯化方法
    Immunoaffinity purified
    免疫原
    A 15 amino acid synthetic peptide derived from the C-terminus of the human and mouse b-catenin protein.

    Type of Immunogen: Synthetic peptide
    亚型
    IgG
    Top Product
    Discover our top product CTNNB1 Primary Antibody
  • 应用备注
    Approved: ELISA (0.1 - 1 μg/mL), IHC, IHC-P (2.5 μg/mL), IP, WB

    Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 μg/mL. Western Blot after IP: 1-2 μg/mL. Cross-reactivity with a ~100kD protein of unknown identity is sometimes observed. Immunoprecipitation: 5-10 μg. Specifically immunoprecipitate the ~92kD b-Catenin protein from native cell lysates. Positive control: HeLa and A431 lysates.
    说明

    Target Species of Antibody: Human

    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    Lot specific
    缓冲液
    PBS,  pH 7.4, 0.1 % sodium azide.
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C,-20 °C
    储存方法
    Short term: 4°C
    Long term: Add glycerol (40-50%) -20°C.
  • 抗原
    CTNNB1 (Catenin (Cadherin-Associated Protein), beta 1, 88kDa (CTNNB1))
    别名
    CTNNB1 / Beta Catenin (CTNNB1 产品)
    别名
    Jup antibody, LOC100217813 antibody, CTNNB1 antibody, CTNNB antibody, Bfc antibody, Catnb antibody, Mesc antibody, ctnnb antibody, id:ibd2058 antibody, wu:fb73e10 antibody, wu:fi81c06 antibody, wu:fk25h01 antibody, ctnnb1 antibody, CHBCAT antibody, MRD19 antibody, armadillo antibody, beta-catenin antibody, catenin beta 1 antibody, catenin (cadherin associated protein), beta 1 antibody, catenin (cadherin-associated protein), beta 1 antibody, catenin beta 1 S homeolog antibody, catenin beta 1 L homeolog antibody, CTNNB1 antibody, Ctnnb1 antibody, ctnnb1 antibody, ctnnb1.S antibody, ctnnb1.L antibody
    背景
    Name/Gene ID: CTNNB1
    Family: Catenin

    Synonyms: CTNNB1, Beta-catenin, Catenin beta-1, CTNNB, Beta Catenin
    基因ID
    1499
    UniProt
    P35222
    途径
    WNT signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Peptide Hormone Metabolism, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Tube Formation, Maintenance of Protein Location, Signaling Events mediated by VEGFR1 and VEGFR2
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