CTNNB1 抗体 (C-Term)
Quick Overview for CTNNB1 抗体 (C-Term) (ABIN337082)
抗原
See all CTNNB1 抗体适用
宿主
克隆类型
标记
应用范围
-
-
抗原表位
- C-Term
-
品牌
- IHC-plus™
-
特异性
- Recognizes human b-catenin. Species cross-reactivity: chicken, mouse, rat and Xenopus.
-
纯化方法
- Immunoaffinity purified
-
免疫原
-
A 15 amino acid synthetic peptide derived from the C-terminus of the human and mouse b-catenin protein.
Type of Immunogen: Synthetic peptide -
亚型
- IgG
-
-
-
-
应用备注
-
Approved: ELISA (0.1 - 1 μg/mL), IHC, IHC-P (2.5 μg/mL), IP, WB
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 μg/mL. Western Blot after IP: 1-2 μg/mL. Cross-reactivity with a ~100kD protein of unknown identity is sometimes observed. Immunoprecipitation: 5-10 μg. Specifically immunoprecipitate the ~92kD b-Catenin protein from native cell lysates. Positive control: HeLa and A431 lysates. -
说明
-
Target Species of Antibody: Human
-
限制
- 仅限研究用
-
-
-
状态
- Liquid
-
浓度
- Lot specific
-
缓冲液
- PBS, pH 7.4, 0.1 % sodium azide.
-
储存液
- Sodium azide
-
注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
储存条件
- 4 °C,-20 °C
-
储存方法
-
Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.
-
-
- CTNNB1 (Catenin (Cadherin-Associated Protein), beta 1, 88kDa (CTNNB1))
-
别名
- CTNNB1 / Beta Catenin
-
背景
-
Name/Gene ID: CTNNB1
Family: Catenin
Synonyms: CTNNB1, Beta-catenin, Catenin beta-1, CTNNB, Beta Catenin -
基因ID
- 1499
-
UniProt
- P35222
-
途径
- WNT signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Peptide Hormone Metabolism, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Tube Formation, Maintenance of Protein Location, Signaling Events mediated by VEGFR1 and VEGFR2
抗原
-