POLL 抗体 (AA 563-575)
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北京 101111
Quick Overview for POLL 抗体 (AA 563-575) (ABIN337014)
抗原
See all POLL 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 563-575
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特异性
- Human POLL. It has been brought to our attention that this product may crossreact with Pol beta (NP_002681), an evolutionarilly related polymerase with a predicted size of 39 kDa. The overall % of identity is not very high (about 32 % in the core) but considering only the peptide that we used as immunogen the two share the sequence YREP**R** at the C-terminus. Further the protein structures appear to be very similar in this region.
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预测反应
- Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Baboon, Monkey, Marmoset, Mouse, Rat, Bovine, Bat, Hamster, Elephant, Panda, Rabbit, Pig (100%) Dog, Opossum, Turkey, Chicken (92%) Xenopus, Zebrafish, Sea anemone (85%).
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纯化方法
- Immunoaffinity purified
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免疫原
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Synthetic peptide C-LGLPYREPAERDW from the C-terminus of human POLL (NP_037406.1). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Baboon, Monkey, Marmoset, Mouse, Rat, Bovine, Bat, Hamster, Elephant, Panda, Rabbit, Pig (100%), Dog, Opossum, Turkey, Chicken (92%), Xenopus, Zebrafish, Sea anemone (85%).
Type of Immunogen: Synthetic peptide
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anti-Polymerase (DNA Directed), lambda (POLL) (C-Term) antibody
Verified POLL 适用: 人 WB, ELISA 宿主: 山羊 Polyclonal unconjugated
anti-Polymerase (DNA Directed), lambda (POLL) (AA 16-211) antibodyPOLL 适用: 人 WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Polymerase (DNA Directed), lambda (POLL) (C-Term) antibodyPOLL 适用: 人, 大鼠, 小鼠 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Polymerase (DNA Directed), lambda (POLL) (AA 525-575) antibodyPOLL 适用: 人, 小鼠 IP, IHC (fp) 宿主: 兔 Polyclonal unconjugated
anti-Polymerase (DNA Directed), lambda (POLL) (AA 525-575) antibodyPOLL 适用: 人, 小鼠 IHC 宿主: 兔 Polyclonal unconjugated
anti-Polymerase (DNA Directed), lambda (POLL) (N-Term) antibodyPOLL 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Polymerase (DNA Directed), lambda (POLL) (C-Term) antibodyPOLL 适用: 人, 大鼠, 小鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Polymerase (DNA Directed), lambda (POLL) (C-Term) antibodyPOLL 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Polymerase (DNA Directed), lambda (POLL) (C-Term) antibodyPOLL 适用: 人, 小鼠, 兔, 豚鼠, Pig, 犬, Bat, Hamster, 猴 WB 宿主: 兔 Polyclonal unconjugated
anti-Polymerase (DNA Directed), lambda (POLL) (AA 421-575) antibodyPOLL 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Approved: IHC, IHC-P (5 μg/mL), Peptide-ELISA (1:32000)
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. -
说明
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Target Species of Antibody: Human
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- Lot specific
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缓冲液
- Tris-buffered saline, pH 7.3, 0.5 % BSA, 0.02 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Minimize freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Store at -20°C. Avoid freeze-thaw cycles.
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- POLL (Polymerase (DNA Directed), lambda (POLL))
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别名
- POLL / DNA Polymerase Lambda
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背景
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Name/Gene ID: POLL
Synonyms: POLL, DNA polymerase beta-2, DNA polymerase beta-N, DNA polymerase lambda, DNA polymerase kappa, Pol beta2, BETAN, Pol Lambda, POLKAPPA -
基因ID
- 27343
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NCBI登录号
- NP_037406
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UniProt
- Q9UGP5
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途径
- DNA Damage Repair, Production of Molecular Mediator of Immune Response
抗原
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