14-3-3 theta 抗体 (N-Term)
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北京 101111
Quick Overview for 14-3-3 theta 抗体 (N-Term) (ABIN336110)
抗原
See all 14-3-3 theta (YWHAQ) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 1-245, N-Term
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特异性
- The antibody recognizes Human 14-3-3 protein theta (tau). Other species not tested.
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产品特性
- Synonyms: 14-3-3 protein tau, 14-3-3 protein T-cell, YWHAQ, Protein HS1
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纯化方法
- Protein-G affinity chromatography
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免疫原
- Recombinant human 14-3-3 tau (aa 1-245) purified from E. coli
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亚型
- IgG1
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应用备注
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ELISA. Western blot (1/1,000-1/2,000). Immunohistochemistry on Paraffin Sections (10 μg/mL). This YWHAQ antibody was validated for use in immunohistochemistry on a panel of 21formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigenretrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides wereincubated with biotinylated secondary antibody, followed by alkalinephosphatase-streptavidin and chromogen.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1.0 mg/mL
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缓冲液
- PBS, pH 7.4, containing 0.09 % Sodium Azide
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C/-20 °C
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储存方法
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Store the antibody undiluted at 2-8 °C for up to two weeks or (in aliquots) at -20 °C or -70 °Cfor longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch. -
有效期
- 12 months
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- 14-3-3 theta (YWHAQ)
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别名
- 14-3-3 Protein theta
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背景
- The 14-3-3 family of proteins plays a key regulatory role in signal transduction, checkpoint control, apoptotic and nutrient-sensing pathways. 14-3-3 proteins are highly conserved and ubiquitously expressed. There are at least seven isoforms, beta, gamma, epsilon, sigma, zeta, tau and eta that have been identified in mammals. The 14-3-3 tau, a subtype of the 14-3-3 family of proteins, was found in T Cells, brain and testes. This 14-3-3 tau is upregulated in patients with amyotrophic lateral sclerosis.Synonyms: 14-3-3 protein T-cell, 14-3-3 protein tau, Protein HS1, YWHAQ
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基因ID
- 10971
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UniProt
- P27348
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途径
- Apoptosis, Myometrial Relaxation and Contraction
抗原
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