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Vimentin 抗体

There is 1 publication for this product available. The 小鼠 单克隆 anti-Vimentin antibody is suitable to detect Vimentin in samples from 人, 大鼠, Pig, 犬, 山羊 和 Hamster. It has been validated for WB, IHC, FACS, ICC 和 IHC (fro).
产品编号 ABIN335381
发货至: 中国
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Quick Overview for Vimentin 抗体 (ABIN335381)

抗原

See all Vimentin (VIM) 抗体
Vimentin (VIM)

适用

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人, 大鼠, Pig, 犬, 山羊, Hamster

宿主

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小鼠

克隆类型

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单克隆

标记

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This Vimentin antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

克隆位点

RV203
  • 特异性

    Human, rat, goat, canine, hamster and swine.

    纯化方法

    Purified

    免疫原

    RV203 is a mouse monoclonal IgG1 antibody derived by fusion of SP2/0-Ag14 mouse myeloma cells with spleen cells from a BALB/c mouse immunized with a cytoskeletal vimentin extract of calf lens.

    亚型

    IgG1
  • 应用备注

    RV203 reacts exclusively with vimentin, which is expressed in mesenchymal cells and mesenchymal derived tumors e.g. lymphoma, sarcoma and melanoma. RV203 is suitable for immunoblotting, immunocytochemistry, immunohistochemistry on frozen tissues and flow cytometry. Optimal antibody dilution should be determined by titration, recommended range is 1:100 - 1:200 for flow cytometry, and for immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, and 1:100 - 1:1000 for immunoblotting applications.

    限制

    仅限研究用
  • 储存条件

    4 °C
  • Schaart, Pieper, Kuijpers, Bloemendal, Ramaekers: "Baby hamster kidney (BHK-21/C13) cells can express striated muscle type proteins." in: Differentiation; research in biological diversity, Vol. 46, Issue 2, pp. 105-15, (1991) (PubMed).

  • 抗原

    Vimentin (VIM)

    别名

    Vimentin

    背景

    Vimentin (57 kDa) is the intermediate filament protein (IFP) of mesenchymal cells. This IFP however often deviates from the tissue-specific and developmentally regulated pattern of expression. Besides its typical expression in most cultured cells, vimentin is also expressed together with several other IFPs during early stages of development. As differentiation proceeds, vimentin is exchanged for the tissue-specific intermediate filament type. Also in cancers, vimentin is often expressed in addition to the tissue-specific IFP.

    途径

    Caspase Cascade in Apoptosis
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