Cardiotin 抗体
There is 1 publication for this product available. The 小鼠 单克隆 anti-Cardiotin antibody is suitable to detect Cardiotin in samples from 人 和 Pig. It has been validated for WB, IHC (fro) 和 IHC.
(1 reference)
产品编号 ABIN335363
发货至:
中国
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Quick Overview for Cardiotin 抗体 (ABIN335363)
抗原
Cardiotin
适用
人, Pig
宿主
小鼠
克隆类型
单克隆
标记
非结合性
应用范围
Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (IHC)
克隆位点
SR-3
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特异性
- Human and swine.
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纯化方法
- Purified
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免疫原
- SR-3 is a mouse monoclonal IgM antibody derived by fusion of SP2/0-Ag14 mouse myeloma cells with spleen cells from a BALB/c mouse immunized with the 100 kDa cardiotin subunit.
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亚型
- IgM
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anti-Cardiotin antibody
适用: 人, Pig, Cat, 犬, 山羊, Hamster, 猴, 小鼠, 大鼠, 非洲爪蟾, 斑马鱼, 兔 WB, IHC (fro), IHC (p), IHC 宿主: 小鼠 Monoclonal R2G unconjugated
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应用备注
- SR-3 reacts with cardiotin, a mitochondrion-associated protein, which is present in cardiomyocytes and skeletal muscle, but also in epithelial cells and tissues. SR-3 is useful for immunohistochemistry on frozen sections and immunoblotting. In immunoblotting assays SR-3 reacts with the 300 kDa cardiotin protein complex and its 100 kDa and 60 kDa subunits. Optimal antibody dilution should be determined by titration, recommended range is 1:100 - 1:200 for immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, and 1:100 - 1:500 for immunoblotting applications.
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限制
- 仅限研究用
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储存条件
- 4 °C
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: "Biochemical characterization of cardiotin, a sarcoplasmic reticulum associated protein." in: FEBS letters, Vol. 403, Issue 2, pp. 168-72, (1997) (PubMed).
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- Cardiotin
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背景
- Cardiotin is a high molecular weight protein complex (300 kDa) located in the mitochondria of cardiomyocytes and skeletal muscle. The cardiotin structure exists of subunits of 60 kDa and 100 kDa, probably in a tetrameric configuration. Both subunits contain the same amino-terminal 14 amino-acid sequence, showing high homology to human skeletal muscle alpha-actinin. During cardiac contractile dysfunction and myocard cell differentiation, the cardiotin distribution is affected. Compared to other structural proteins, cardiotin is one of the first to respond to insults (ischemia, fibrillation) that influence the functional status of cardiomyocytes.
抗原
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