Cardiotin 抗体
适用: 人, Pig, Cat, 犬, 山羊, Hamster, 猴, 大鼠, 非洲爪蟾, 小鼠, 斑马鱼, 兔
WB, IHC (fro), IHC, IHC (p)
宿主: 小鼠
Monoclonal
R2G
unconjugated
4 images
(6 references)-
- 抗原
- Cardiotin
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适用
- 人, Pig, Cat, 犬, 山羊, Hamster, 猴, 大鼠, 非洲爪蟾, 小鼠, 斑马鱼, 兔
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- 非结合性
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应用范围
- Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 特异性
- Human, monkey, swine, feline, canine, goat, hamster, mouse, rat, rabbit and Xenopus.
- 纯化方法
- Purified
- 免疫原
- R2G is a mouse monoclonal IgM antibody derived by fusion of SP2/0-Ag14 mouse myeloma cells, with spleen cells from a mouse immunized with a total protein extract of chicken gizzard.
- 克隆位点
- R2G
- 亚型
- IgM
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anti-Cardiotin antibody
适用: 人, Pig, Cat, 犬, 山羊, Hamster, 猴, 大鼠, 非洲爪蟾, 兔 WB, IHC (fro), IHC 宿主: 小鼠 Monoclonal R2G unconjugated
anti-Cardiotin antibody适用: 人, Pig WB, IHC (fro), IHC, FACS 宿主: 小鼠 Monoclonal SR-4 unconjugated
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- 应用备注
- R2G reacts with cardiotin, a mitochondrion-associated protein, which is present in cardiomyocytes and skeletal muscle. R2G is useful for immunohistochemistry on frozen and paraffin-embedded tissue and immunoblotting. In immunoblotting assays R2G reacts with the 300 kDa cardiotin protein complex and its 100 kDa and 60 kDa subunits. Recommended range is 1:25 - 1:100 for immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent. Optimal antibody dilution for immunoblotting applications should be determined by titration.
- 限制
- 仅限研究用
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- 储存条件
- 4 °C
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: "Rat adult stem cells (marrow stromal cells) engraft and differentiate in chick embryos without evidence of cell fusion." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, Issue 25, pp. 9282-5, (2004) (PubMed).
: "Time course of atrial fibrillation-induced cellular structural remodeling in atria of the goat." in: Journal of molecular and cellular cardiology, Vol. 33, Issue 12, pp. 2083-94, (2001) (PubMed).
: "Adult rabbit cardiomyocytes undergo hibernation-like dedifferentiation when co-cultured with cardiac fibroblasts." in: Cardiovascular research, Vol. 51, Issue 2, pp. 230-40, (2001) (PubMed).
: "Dedifferentiation of atrial cardiomyocytes as a result of chronic atrial fibrillation." in: The American journal of pathology, Vol. 151, Issue 4, pp. 985-97, (1997) (PubMed).
: "Biochemical characterization of cardiotin, a sarcoplasmic reticulum associated protein." in: FEBS letters, Vol. 403, Issue 2, pp. 168-72, (1997) (PubMed).
: "Characterization of cardiotin, a structural component in the myocard." in: European journal of cell biology, Vol. 62, Issue 1, pp. 34-48, (1994) (PubMed).
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: "Rat adult stem cells (marrow stromal cells) engraft and differentiate in chick embryos without evidence of cell fusion." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, Issue 25, pp. 9282-5, (2004) (PubMed).
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- 抗原
- Cardiotin
- 背景
- Cardiotin is a high molecular weight protein complex (300 kDa) located in the mitochondria of cardiomyocytes and skeletal muscle. The cardiotin structure exists of subunits of 60 kDa and 100 kDa, probably in a tetrameric configuration. Both subunits contain the same amino-terminal 14 amino-acid sequence, showing high homology to human skeletal muscle alpha-actinin. During cardiac contractile dysfunction and myocard cell differentiation, the cardiotin distribution is affected. Compared to other structural proteins, cardiotin is one of the first to respond to insults (ischemia, fibrillation) that influence the functional status of cardiomyocytes.
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