Cardiotin 抗体
This 小鼠 单克隆 antibody specifically detects Cardiotin in WB, IHC (fro), IHC (p) 和 IHC. It exhibits reactivity toward 人 和 Pig.It has been mentioned in 1 publication
2 images
(1 reference)
产品编号 ABIN335362
发货至:
中国
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Quick Overview for Cardiotin 抗体 (ABIN335362)
抗原
Cardiotin
适用
人, Pig
宿主
小鼠
克隆类型
单克隆
标记
非结合性
应用范围
Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC)
克隆位点
SR-2
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特异性
- Human and swine.
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纯化方法
- Purified
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免疫原
- SR-2 is a mouse monoclonal IgM antibody derived by fusion of SP2/0-Ag14 mouse myeloma cells with spleen cells from a BALB/c mouse immunized with the 100 kDa cardiotin subunit.
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亚型
- IgM
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anti-Cardiotin antibody
适用: 人, Pig, Cat, 犬, 山羊, Hamster, 猴, 小鼠, 大鼠, 非洲爪蟾, 斑马鱼, 兔 WB, IHC (fro), IHC (p), IHC 宿主: 小鼠 Monoclonal R2G unconjugated
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应用备注
- SR-2 reacts with cardiotin, a mitochondrion-associated protein, which is present in cardiomyocytes and skeletal muscle. SR-2 reacts with cardiomyocytes, skeletal muscle, stromal and epithelial cells as well in vivo as in vitro. SR-2 is useful for immunohistochemistry on frozen and paraffin-embedded tissue and immunoblotting. In immunoblotting assays SR-2 reacts with the 300 kDa cardiotin protein complex and its 100 kDa and 60 kDa subunits. Optimal antibody dilution should be determined by titration, recommended range is 1:25 - 1:100 for immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, and 1:50 - 1:500 for immunoblotting applications.
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限制
- 仅限研究用
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储存条件
- 4 °C
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: "Biochemical characterization of cardiotin, a sarcoplasmic reticulum associated protein." in: FEBS letters, Vol. 403, Issue 2, pp. 168-72, (1997) (PubMed).
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- Cardiotin
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背景
- Cardiotin is a high molecular weight protein complex (300 kDa) located in the mitochondria of cardiomyocytes and skeletal muscle. The cardiotin structure exists of subunits of 60 kDa and 100 kDa, probably in a tetrameric configuration. Both subunits contain the same amino-terminal 14 amino-acid sequence, showing high homology to human skeletal muscle alpha-actinin. During cardiac contractile dysfunction and myocard cell differentiation, the cardiotin distribution is affected. Compared to other structural proteins, cardiotin is one of the first to respond to insults (ischemia, fibrillation) that influence the functional status of cardiomyocytes.
抗原
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