KRT20 抗体
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北京 101111
Quick Overview for KRT20 抗体 (ABIN335316)
抗原
See all KRT20 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Mouse anti Cytokeratin 20
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特异性
- MKs20-8 may be used as a marker for certain types of carcinomas such as adenocarcinomas of the colon, transitional cell carcinomas of the bladder and Merkel cell tumors of the skin, it may also be used as a sensitive detection of intestinal and gastric foveolar epithelium, urothelial umbrella cells, Merkel cells of epidermis as well as tumors originating therefrom (e.g. primary and metastatic colorectal carcinoma).
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产品特性
- Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in Human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40 kDa and 68 kDa and isoelectric pH between 4.9 - 7.8. The individual Human Cytokeratins are numbered 1 to 20. The various epithelia in the Human body usually express Cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of matuRation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The Cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.
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纯化方法
- culture supernatant
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免疫原
- MKs20-8 is a Mouse monoclonal IgG2a antibody derived by fusion of Mouse myeloma cells with spleen cells from a Balb/c Mouse immunized with electrophoretically purified Keratin K20 from Human intestinal mucosa.
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亚型
- IgG2a
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anti-Keratin 20 (KRT20) antibody
KRT20 适用: 人 WB, IHC (p), IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Keratin 20 (KRT20) (AA 245-424) antibodyKRT20 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Keratin 20 (KRT20) (AA 1-424) antibodyKRT20 适用: 人 WB, ELISA, IHC (p), IF 宿主: 小鼠 Monoclonal 2G3-1C8 unconjugated
anti-Keratin 20 (KRT20) (AA 196-323) antibodyKRT20 适用: 人 WB, IHC 宿主: 小鼠 Monoclonal KRT20-1992 unconjugated
anti-Keratin 20 (KRT20) (AA 196-323) antibodyKRT20 适用: 人 WB, IHC 宿主: 小鼠 Monoclonal KRT20-1991 unconjugated
anti-Keratin 20 (KRT20) (AA 29-431) antibodyKRT20 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC, FACS 宿主: 兔 Polyclonal unconjugated
anti-Keratin 20 (KRT20) (AA 196-323) antibodyKRT20 适用: 人 WB, IHC 宿主: 兔 Monoclonal KRT20-3129R unconjugated Recombinant Antibody
anti-Keratin 20 (KRT20) (AA 196-323) antibodyKRT20 适用: 人 WB, IHC 宿主: 小鼠 Monoclonal KRT20-3145 unconjugated
anti-Keratin 20 (KRT20) (AA 1-150) antibodyKRT20 适用: 人 IHC, ELISA 宿主: 小鼠 Monoclonal 2C7B9 unconjugated
anti-Keratin 20 (KRT20) antibodyKRT20 适用: 人 WB, IHC (p), IF, ICC, FACS, IHC (fro) 宿主: 兔 Monoclonal unconjugated Recombinant Antibody
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应用备注
- MKs20-8 is suitable for immunoprecipitation, immunoblotting, immunocytochemistry on fixed cells, and immunohistochemistry on frozen and paraffin-embedded tissues. Optimal antibody dilution should be determined by titration.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- Each vial contains 1 mL of culture supernatant of monclonal antibody containing 0.09 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- The antibody is shipped at ambient temperature and may be stored at +4°C. For prolonged storage prepare appropriate aliquots and store at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance or the concentration of the product.
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: "Identification of protein IT of the intestinal cytoskeleton as a novel type I cytokeratin with unusual properties and expression patterns." in: The Journal of cell biology, Vol. 111, Issue 2, pp. 567-80, (1990) (PubMed).
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- KRT20 (Keratin 20 (KRT20))
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别名
- Cytokeratin 20 / Keratin K20
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UniProt
- P35900
抗原
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