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EWSR1 抗体 (Internal Region)

The 山羊 多克隆 anti-EWSR1 antibody is suitable to detect EWSR1 in samples from 人. It has been validated for WB, ELISA 和 IHC.
产品编号 ABIN335135
发货至: 中国
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Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for EWSR1 抗体 (Internal Region) (ABIN335135)

抗原

See all EWSR1 抗体
EWSR1 (Ewing Sarcoma Breakpoint Region 1 (EWSR1))

适用

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宿主

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山羊

克隆类型

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多克隆

标记

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This EWSR1 antibody is un-conjugated

应用范围

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Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

质量等级

Verified
  • 抗原表位

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    Internal Region

    原理

    EWS / EWSR1

    序列

    TSYDQSSYSQ QNTYG

    特异性

    This antibody is expected to recognize all five reported isoforms (NP_053733.2, NP_005234.1, NP_001156757.1, NP_001156758.1, NP_001156759.1).

    交叉反应

    Cow, 人, 小鼠, Pig, 大鼠

    纯化方法

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

    免疫原

    Peptide with sequence C-TSYDQSSYSQQNTYG, from the internal region of the protein sequence according to NP_053733.2, NP_005234.1, NP_001156757.1, NP_001156758.1, NP_001156759.1.

    亚型

    IgG
  • 应用备注

    Immunohistochemistry: Paraffin embedded Human Kidney. Recommended concentration: 5-7 μg/mL.

    Western Blot: Approx. 90 kDa band observed in nuclear lysates of cell line HeLa (calculated MW of 68.5 kDa according to NP_053733.1). This molecular weight is routinely observed by other sources. Recommended concentration: 0.3-1 μg/mL.

    Peptide ELISA: antibody detection limit dilution 1:64000.

    说明

    Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    0.5 mg/mL

    缓冲液

    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Minimize freezing and thawing.

    储存条件

    -20 °C

    储存方法

    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • 抗原

    EWSR1 (Ewing Sarcoma Breakpoint Region 1 (EWSR1))

    别名

    EWSR1

    背景

    EWSR1, EWS, Ewing sarcoma breakpoint region 1, EWS/FLI fusion protein, EWSR1/ZNF384 fusion protein, Ewings sarcoma EWS-Fli1 (type 1) oncogene, bK984G1.4 (Ewing sarcoma breakpoint region 1 protein), extraskeletal myxoid chondrosarcoma EWS/TEC/CHN fusion pr

    基因ID

    2130, 14030, 289752

    NCBI登录号

    NP_053733, NP_005234, NP_001156757, NP_001156758, NP_001156759
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