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ZEB1 抗体 (Internal Region)

This anti-ZEB1 antibody is a 山羊 多克隆 antibody detecting ZEB1 in WB, ELISA 和 IF. Suitable for 人 和 小鼠.
产品编号 ABIN335118
发货至: 中国

Quick Overview for ZEB1 抗体 (Internal Region) (ABIN335118)

抗原

See all ZEB1 抗体
ZEB1 (Zinc Finger E-Box Binding Homeobox 1 (ZEB1))

适用

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人, 小鼠

宿主

  • 34
  • 14
山羊

克隆类型

  • 29
  • 19
多克隆

标记

  • 44
  • 2
  • 1
  • 1
This ZEB1 antibody is un-conjugated

应用范围

  • 39
  • 20
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Western Blotting (WB), ELISA, Immunofluorescence (IF)

质量等级

Verified
  • 抗原表位

    • 5
    • 5
    • 4
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    • 2
    • 2
    • 1
    • 1
    • 1
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    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Internal Region

    原理

    AREB6 / ZEB1

    序列

    RPQIRQKIEN KPLQE

    特异性

    This antibody is expected to recognize both reported isoforms (NP_001121600.1, NP_110378.3).

    交叉反应

    人, 小鼠, 大鼠

    纯化方法

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

    免疫原

    Peptide with sequence C-RPQIRQKIENKPLQE, from the internal region of the protein sequence according to NP_001121600.1, NP_110378.3.

    亚型

    IgG
  • 应用备注

    Western Blot: Approx.100 kDa band observed in Mouse Heart lysates (calculated MW of 122 kDa according to Human NP_110378.3 and Mouse NP_035676.1). Recommended concentration: 0.05-0.2 μg/mL. Primary incubation was 1 hour. Preliminary testing was unsuccessful

    Peptide ELISA: antibody detection limit dilution 1:32000.

    说明

    Immunofluorescence: Strong expression of the protein seen in the nuclei and cytoplasm of U2OS cells. Recommended concentration: 10μg/ml.

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    0.5 mg/mL

    缓冲液

    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Minimize freezing and thawing.

    储存条件

    -20 °C

    储存方法

    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • 抗原

    ZEB1 (Zinc Finger E-Box Binding Homeobox 1 (ZEB1))

    别名

    ZEB1

    背景

    ZEB1, AREB6, zinc finger E-box binding homeobox 1, BZP, DELTA-EF1, MGC133261, NIL-2-A, NIL-2A, NIL2A, TCF8, ZEB, ZFHEP, ZFHX1A, OTTHUMP00000019405, transcription factor 8 (represses interleukin 2 expression), zinc finger homeodomain enhancer-binding prote

    基因ID

    6935, 21417, 25705

    NCBI登录号

    NP_001121600, NP_110378

    途径

    Regulation of Muscle Cell Differentiation
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