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MBD2 抗体 (Internal Region)

Verified MBD2 适用: 人 WB, ELISA, IHC 宿主: 山羊 Polyclonal unconjugated
产品编号 ABIN334432
发货至: 中国
  • 抗原 See all MBD2 抗体
    MBD2 (Methyl-CpG Binding Domain Protein 2 (MBD2))
    抗原表位
    • 10
    • 8
    • 7
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Internal Region
    适用
    • 48
    • 24
    • 6
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    宿主
    • 46
    • 2
    山羊
    克隆类型
    • 45
    • 3
    多克隆
    标记
    • 26
    • 4
    • 3
    • 3
    • 3
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This MBD2 antibody is un-conjugated
    应用范围
    • 41
    • 21
    • 5
    • 5
    • 4
    • 4
    • 2
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
    原理
    MBD2 (isoform 1)
    序列
    RNDPLNQNKG KPDLN
    特异性
    This antibody is expected to resognize isoform 1 (NP_003918.1) only.
    交叉反应
    犬, 人, 小鼠, 大鼠
    纯化方法
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    质量等级
    Verified
    免疫原
    Peptide with sequence C-RNDPLNQNKGKPDLN, from the internal region of the protein sequence according to NP_003918.1.
    亚型
    IgG
  • 应用备注

    Immunohistochemistry: Paraffin embedded Human Colon, Adrenal Gland and Skin. Recommended concentration: 2.5 μg/mL.

    Western Blot: Approx 48-50 kDa band observed in lysates of cell line Jurkat (calculated MW of 43.3 kDa according to NP_003918.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Tan et al, Mol Cell Bio

    Peptide ELISA: antibody detection limit dilution 1:64000.

    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    0.5 mg/mL
    缓冲液
    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    注意事项
    Minimize freezing and thawing.
    储存条件
    -20 °C
    储存方法
    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Bartels, Spruijt, Brinkman, Jansen, Vermeulen, Stunnenberg: "A SILAC-based screen for Methyl-CpG binding proteins identifies RBP-J as a DNA methylation and sequence-specific binding protein." in: PLoS ONE, Vol. 6, Issue 10, pp. e25884, (2012) (PubMed).

    Spruijt, Bartels, Brinkman, Tjeertes, Poser, Stunnenberg, Vermeulen: "CDK2AP1/DOC-1 is a bona fide subunit of the Mi-2/NuRD complex." in: Molecular bioSystems, Vol. 6, Issue 9, pp. 1700-6, (2010) (PubMed).

  • 抗原
    MBD2 (Methyl-CpG Binding Domain Protein 2 (MBD2))
    别名
    MBD2 (MBD2 产品)
    别名
    MBD2 antibody, DMTase antibody, NY-CO-41 antibody, MBD2a antibody, wu:fa97f06 antibody, zgc:111862 antibody, methyl-CpG binding domain protein 2 antibody, methyl-CpG binding domain protein 2 S homeolog antibody, MBD2 antibody, Mbd2 antibody, mbd2 antibody, mbd2.S antibody
    背景
    MBD2, methyl-CpG binding domain protein 2 , DKFZp586O0821, DMTase, NY-CO-41
    基因ID
    8932, 17191
    NCBI登录号
    NP_003918
    途径
    Chromatin Binding
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