MBD2 抗体 (Internal Region)
(2 references)Quick Overview for MBD2 抗体 (Internal Region) (ABIN334432)
抗原
See all MBD2 抗体适用
宿主
克隆类型
标记
应用范围
质量等级
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抗原表位
- Internal Region
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原理
- MBD2 (isoform 1)
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序列
- RNDPLNQNKG KPDLN
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特异性
- This antibody is expected to resognize isoform 1 (NP_003918.1) only.
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交叉反应
- 犬, 人, 小鼠, 大鼠
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纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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免疫原
- Peptide with sequence C-RNDPLNQNKGKPDLN, from the internal region of the protein sequence according to NP_003918.1.
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亚型
- IgG
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anti-Methyl-CpG Binding Domain Protein 2 (MBD2) (AA 250-279) antibody
MBD2 适用: 人 WB, IF 宿主: 兔 Polyclonal RB41663 unconjugated
anti-Methyl-CpG Binding Domain Protein 2 (MBD2) (N-Term) antibodyMBD2 适用: 人, 小鼠, 大鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Methyl-CpG Binding Domain Protein 2 (MBD2) (AA 145-411) antibodyMBD2 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Methyl-CpG Binding Domain Protein 2 (MBD2) (AA 10-228) antibodyMBD2 适用: 人 WB, IHC (p) 宿主: 小鼠 Monoclonal 1812CT625-9-45 unconjugated
anti-Methyl-CpG Binding Domain Protein 2 (MBD2) (AA 202-411) antibodyMBD2 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Methyl-CpG Binding Domain Protein 2 (MBD2) antibodyMBD2 适用: 人, 小鼠, 大鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Methyl-CpG Binding Domain Protein 2 (MBD2) (Middle Region) antibodyMBD2 适用: 人, 小鼠, 大鼠, 犬, Cow, 豚鼠, 马, 兔, 斑马鱼 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Methyl-CpG Binding Domain Protein 2 (MBD2) (Center) antibodyMBD2 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Methyl-CpG Binding Domain Protein 2 (MBD2) (N-Term) antibodyMBD2 适用: 人, 小鼠, 大鼠, Bat, 猴 WB 宿主: 兔 Polyclonal unconjugated
anti-Methyl-CpG Binding Domain Protein 2 (MBD2) (AA 361-411) antibodyMBD2 适用: 人, 小鼠, 犬, 豚鼠, Pig WB, IHC, IP, IF, IHC (p) 宿主: 兔 Polyclonal unconjugated
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应用备注
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Immunohistochemistry: Paraffin embedded Human Colon, Adrenal Gland and Skin. Recommended concentration: 2.5 μg/mL.
Western Blot: Approx 48-50 kDa band observed in lysates of cell line Jurkat (calculated MW of 43.3 kDa according to NP_003918.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Tan et al, Mol Cell Bio
Peptide ELISA: antibody detection limit dilution 1:64000.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.5 mg/mL
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缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Minimize freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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: "A SILAC-based screen for Methyl-CpG binding proteins identifies RBP-J as a DNA methylation and sequence-specific binding protein." in: PLoS ONE, Vol. 6, Issue 10, pp. e25884, (2012) (PubMed).
: "CDK2AP1/DOC-1 is a bona fide subunit of the Mi-2/NuRD complex." in: Molecular bioSystems, Vol. 6, Issue 9, pp. 1700-6, (2010) (PubMed).
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: "A SILAC-based screen for Methyl-CpG binding proteins identifies RBP-J as a DNA methylation and sequence-specific binding protein." in: PLoS ONE, Vol. 6, Issue 10, pp. e25884, (2012) (PubMed).
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- MBD2 (Methyl-CpG Binding Domain Protein 2 (MBD2))
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别名
- MBD2
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背景
- MBD2, methyl-CpG binding domain protein 2 , DKFZp586O0821, DMTase, NY-CO-41
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基因ID
- 8932, 17191
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NCBI登录号
- NP_003918
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途径
- Chromatin Binding
抗原
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