ERCC1 抗体 (N-Term)
Quick Overview for ERCC1 抗体 (N-Term) (ABIN334392)
抗原
See all ERCC1 抗体适用
宿主
克隆类型
标记
应用范围
质量等级
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抗原表位
- N-Term
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原理
- ERCC1
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序列
- DPGKDKEGVP QPS
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特异性
- This antibody is expected to recognise both reported isoforms (NP_973730.1 and NP_001974.1).
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交叉反应
- 人
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纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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免疫原
- Peptide with sequence DPGKDKEGVPQPS-C, from the N Terminus of the protein sequence according to NP_973730.1, NP_001974.1.
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亚型
- IgG
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anti-Excision Repair Cross Complementing Polypeptide-1 (ERCC1) (AA 151-250) antibody
ERCC1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Excision Repair Cross Complementing Polypeptide-1 (ERCC1) (AA 1-297) antibodyERCC1 适用: 人, 小鼠 WB, ELISA, IHC, FACS 宿主: 小鼠 Monoclonal 5A2D1 unconjugated
anti-Excision Repair Cross Complementing Polypeptide-1 (ERCC1) (AA 191-281) antibodyERCC1 适用: 人 IHC, StM 宿主: 小鼠 Monoclonal ERCC1-2318 unconjugated
anti-Excision Repair Cross Complementing Polypeptide-1 (ERCC1) (AA 151-297) antibodyERCC1 适用: 人 ELISA, FACS, ICC 宿主: 小鼠 Monoclonal 1E5B3 unconjugated
anti-Excision Repair Cross Complementing Polypeptide-1 (ERCC1) (AA 1-120) antibodyERCC1 适用: 人 ELISA, FACS 宿主: 小鼠 Monoclonal 3F9E9 unconjugated
anti-Excision Repair Cross Complementing Polypeptide-1 (ERCC1) (AA 1-323) antibodyERCC1 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Excision Repair Cross Complementing Polypeptide-1 (ERCC1) (AA 207-281) antibodyERCC1 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 3A7 unconjugated
anti-Excision Repair Cross Complementing Polypeptide-1 (ERCC1) (Internal Region) antibodyERCC1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Excision Repair Cross Complementing Polypeptide-1 (ERCC1) (Internal Region) antibodyERCC1 适用: 人, 小鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Excision Repair Cross Complementing Polypeptide-1 (ERCC1) (AA 1-323) antibodyERCC1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western Blot: Approx 38 kDa band observed in lysates of cell lines A431 and Kelly (calculated MW of 35.6 kDa according to NP_973730.1). This molecular weight is routinely observed by other sources. Recommended concentration: 0.3-1 μg/mL.
Peptide ELISA: antibody detection limit dilution 1:64000.
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说明
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Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.5 mg/mL
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缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Minimize freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- ERCC1 (Excision Repair Cross Complementing Polypeptide-1 (ERCC1))
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别名
- ERCC1
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背景
- ERCC1, excision repair cross-complementing rodent repair deficiency, complementation group 1 (includes overlapping antisense sequence), COFS4, UV20, excision repair cross-complementing 1, excision repair protein
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基因ID
- 2067
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NCBI登录号
- NP_973730, NP_001974
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途径
- DNA Damage Repair, Production of Molecular Mediator of Immune Response
抗原
-
