ARF1 抗体 (C-Term)
Quick Overview for ARF1 抗体 (C-Term) (ABIN334352)
抗原
See all ARF1 抗体适用
宿主
克隆类型
标记
应用范围
质量等级
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抗原表位
- C-Term
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原理
- ARF1 (variants 1-4)
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序列
- EGLDWLSNQL RNQK
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特异性
- This antibody is expected to recognize all four reported isoforms.
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交叉反应
- 犬, 人, 小鼠, 大鼠
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纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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免疫原
- Peptide with sequence C-EGLDWLSNQLRNQK, from the C Terminus of the protein sequence according to NP_001019397.1, NP_001649.1, NP_001019398.1, NP_001019399.1.
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亚型
- IgG
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应用备注
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Western Blot: Approx 18 kDa band observed in lysates of cell lines A431, HeLa, MCF7, NIH3T3, KNRK and MDCK (calculated MW of 20.7 kDa according to Human NP_001649.1, Mouse NP_031502.1 and Rat NP_071963.1). Recommended concentration: 0.3-1 μg/mL. Primary inc
Peptide ELISA: antibody detection limit dilution 1:8000.
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说明
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Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: 10ug/ml.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.5 mg/mL
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缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Minimize freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- ARF1 (ADP-Ribosylation Factor 1 (ARF1))
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别名
- ARF1
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背景
- ARF1, ARF 1, ADP-ribosylation factor 1, ADP ribosylation factor 1
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基因ID
- 375, 376, 377
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NCBI登录号
- NP_001019397, NP_001649, NP_001019398, NP_001019399
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途径
- Transition Metal Ion Homeostasis, Inositol Metabolic Process
抗原
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