EEF2 抗体 (pThr56)
Quick Overview for EEF2 抗体 (pThr56) (ABIN334266)
抗原
See all EEF2 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- pThr56
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特异性
- Detects endogenous levels of eEF2 only when phosphorylated at threonine 56. It does not recognize eEF2 phosphorylated at other sites.
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纯化方法
- Immunoaffinity purified
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免疫原
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Synthetic phosphopeptide (KLH coupled) corresponding to residues surrounding Thr56 of human eEF2.
Type of Immunogen: Synthetic phospho-peptide -
亚型
- IgG
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anti-Eukaryotic Translation Elongation Factor 2 (EEF2) (AA 32-233) antibody
EEF2 适用: 人 WB, IHC, IF, FACS 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Elongation Factor 2 (EEF2) antibodyEEF2 适用: 人 WB, IHC, IF 宿主: 兔 Monoclonal unconjugated
anti-Eukaryotic Translation Elongation Factor 2 (EEF2) antibodyEEF2 适用: 人 WB, IHC, ELISA, ICC 宿主: 小鼠 Monoclonal 5B6 unconjugated
anti-Eukaryotic Translation Elongation Factor 2 (EEF2) antibodyEEF2 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Elongation Factor 2 (EEF2) (AA 1-290) antibodyEEF2 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Elongation Factor 2 (EEF2) (AA 2-858) antibodyEEF2 适用: 人 IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Elongation Factor 2 (EEF2) (AA 36-85) antibodyEEF2 适用: 人, 小鼠, 大鼠, 斑马鱼, Cow, 小鸡, 猴, 犬, Pig, 非洲爪蟾, 豚鼠, 马, Hamster, Drosophila melanogaster, Bat WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Elongation Factor 2 (EEF2) (Ser311) antibodyEEF2 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Elongation Factor 2 (EEF2) (N-Term) antibodyEEF2 适用: 人, 小鼠, 大鼠, Drosophila melanogaster, C. elegans WB 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Elongation Factor 2 (EEF2) (N-Term), (pSer56) antibodyEEF2 适用: 人, 小鼠, 大鼠, 斑马鱼, Cow, 小鸡, 猴 WB, IHC, IF, IC 宿主: 兔 Polyclonal unconjugated
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应用备注
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Approved: ELISA, IHC, IHC-P (1:100), WB (1:1000)
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 1:100. Western Blot: 1:1000, Incubate membrane with diluted antibody in 5 % BSA, 1X TBS, 0.1 % Tween-20 at 4uC with gentle shaking, overnight. -
说明
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Target Species of Antibody: Human
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- Lot specific
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缓冲液
- 10 mM HEPES, pH 7.5, 150 mM sodium chloride, 0.1 mg/mL BSA, 50 % glycerol. No preservative added.
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储存液
- Without preservative
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注意事项
- Avoid repeat freeze-thaw cycles.
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储存条件
- 4 °C,-20 °C
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储存方法
- Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
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- EEF2 (Eukaryotic Translation Elongation Factor 2 (EEF2))
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别名
- EEF2 / Elongation Factor 2
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背景
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Name/Gene ID: EEF2
Synonyms: EEF2, EEF-2, EF-2, Elongation factor 2, Polypeptidyl-tRNA translocase, EF2 -
基因ID
- 1938
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UniProt
- P13639
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途径
- AMPK Signaling
抗原
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